Isolation and survey on antibiotic resistance of H. pylori

In order to determine of Helicobacter pylori antibiotic resistance diagnosis and susceptibility profile directs the applicability of recommended treatment regimens in our setting. 100 dyspeptic adult patients undergoing endoscopy from a specific gastroenterology clinic in Rasht city. Multiple gastric biopsy and specimens were submitted for gram stain, culture, antimicrobial sensitivity testing, rapid urease test and histology. Antimicrobial susceptibility testing was done by MIC method against metronidazole, clarithromycin, amoxicillin, and tetracycline. Forty percent (40%) of the study population was positive for H. pylori infection, 60% was males. H. pylori culture showed a sensitivity of 45%, specificity of 98%. All H. pylori strains isolated were sensitive to metronidazole, clarithromycin, amoxicillin and tetracycline. Helicobacter pylori diagnosis and susceptibility profile directs the applicability of recommended treatment regimens in our setting. Knowledge of the antibiotic susceptibility patterns in our setting allows us to be more cautious in the choice of first-line agents. Information on antibiotic susceptibility profile plays an important role in empiric antibiotic treatment and management of refractive cases.