Enzyme Assay of Aeluropus littoralis Parl. Regarding to the Salt (NaCl) Stresses

Reactive oxygen species (ROS) release from cellular water molecules under abiotic andbiotic stresses which are harmful for cellular metabolism. Although, some of radicalinjuries neutralize via plant enzymatic and nonenzymatic reactions like antioxidant systemsuch as superoxide dismutase, catalase, peroxidase and ascorbate peroxidase. Aeluropuslittoralis Parl. is a halophyte plant belongs to Poaceae family can tolerate over 600 mMNaCl stress, thus it can be as genetic source for transfer gene to crop resulting cerealimprovement under salt stress condition. There is not any report about antioxidant assay inthis plant up to now. This research have studied in rice and citrus research institute, SariAgricultural Sciences and Natural Resources University’s hydroponic labs in 1389.Antioxidant enzymes assay have performed in four levels, 0, 200, 400 and 600 mM NaClduring 21 days and periodically 72 hours sampling. Statistical analysis indicated reciprocaleffects of time and salt treatment for enzyme was significant (á= 0.01). The resultsdetermined the enzymes activity increase up to 8, 7, 18 and 5.3 for POX, CAT, APX andSOD respectively in 400 and 600 mM NaCl treatment. In respect to results as well asantioxidant enzymes high activity, identification, isolation and transfer of genes involve insalt tolerance from Aeluropus littoralis Parl. can be benefit for production of transgenicplant tolerant to environmental stresses.