Effect of Inoculation of Heavy Metal-Resistant Soil Microbes on Growth and Uptake of Lead and Cadmium by Three Pasture Crops

Arbuscular mycorrhizae (AM) and Plant Growth Promoting Rhizobacteria (PGPR) associations are integral and functioning parts of plant roots. These associations have a basic role in root uptake efficiency as well as improvement of plant growth in degraded environments including heavy metals contaminated soils. This study was conducted to evaluate the effects of heavy metal-resistant soil microbe's inoculation on bio-availability of Pb and Cd in soil, plant growth as well as metal uptake by Millet (Pennisetum glaucum), Couch grass (Triticum repens) and wild alfalfa (Medicago sativa). A soil sample was treated by different levels of Pb and Cd (soil 1). Native microbial inoculums were obtained from alfalfa rhizosphere soils adjacent to Pb and Cd mines in Zanjan region (soil 2), then added with weight ratio of 1:5 (w/w) to soil 1. Host plants including millet, couch grass, and alfalfa were grown in pots and kept in greenhouse conditions. At the end of growing period, shoot dry matter and Pb and Cd concentrations in plant and soil were measured. Results indicated that plants yield and Pb uptake were significantly higher in non-inoculated treatments (p ≤ 0.05). However, Cd uptake by plants was greater in inoculated treatments (p ≤ 0.05). Couch grass showed the most accumulation potential of Cd and Pb among the studied plants.