Investigation on Inheritance of Glyphosate tolerance in T1 Generation of Transgenic Rapeseed (Brassica napus L.) with the mutated bacterial EPSPS gene

In transgenic plants، the faithful inheritance and expression of transgenes are particularly important properties. In this study analyses were performed on seeds that produced by T0 glyphosate-tolerant rapeseed plants. The T0 generation plants contains a mutated EPSPS gene that has a highly linked with nptII gene. These seed screened on MS medium containing 15 mgL-1 kanamycin and explants with green leaves and expanded roots considered as tolerant plants. Results showed that mutated EPSPS gene expresses in T1 canola plants decrease the harmful consequences of glyphosate. The statistical 2 analysis (p-value 0. 05) revealed that the segregation of the transgenes to T1 progeny suggested a Mendelian pattern as single dominant locus. In molecular analysis، the presence and transcription of EPSPS genes studied by PCR and RT-PCR، respectively. The T1 plants were fully fertile، with no apparent phenotypic abnormalities.