Callusogenesis investigation of lemon balm (Melissa officinalis L.)

In this research callus induction under in vitro condition in medicinal plant, lemon balm (Melissa officinalis L.) has been considered as a preliminary method for modification of this plant. For this purpose factorial expriment based on completely randomize design has been used with factors, ecotype (Isfahan and Karaj), origin of explant (leaf, petiol, internode), Lighting conditions (light and dark), 2, 4-D (0, 0.5, 1, 2 mg/l) and BAP (0, 1, 2 mg/l) with three replications. Results showed that the best hormonal treatment for callus induction in lemon balm was 1mg/l 2, 4-D + 1mg/l BAP for internodes and petiole in light condition. After that, use of leaf explants with 0.5mg/l 2, 4-D + 1mg/l BAP in dark and then light condition was the best.