Effect of low level laser therapy with the application of DFDBA in rabbits

Low Level Laser Therapy is used in many aspects in medicine and dentistry, such as treatment of bone lesions. The purpose of this study was to evaluate the effect of low level laser (GaAlAs) combined with the application of DFDBA in bone healing of rabbit tibia. The experimental study was done on 12 male albino rabbits with an approximate weight of 1.5 kg and about 6 months of age. In order to perform the surgery, animals were anesthetized with intravenous injection of Ketamin 10% and Xylazine 2%. An artificial lesion was created using implant drills (height of 4 mm and diameter of 2.8 mm) in tibia bone and was filled with DFDBA. Then, the lesions were irradiated using laser with distinct protocol (10 days irradiation, 14 days rest, 10 days irradiation, 14 days rest). Type of the laser was GaAlAr with wavelength of 808 nm, energy of 6 J/cm2 and in continuous mode. No irradiation was done in the control group. After 48 days, the animals were sacrificed and sections from tibia was made and the specimens were underwent histological assessments. Wilcoxon signed rank test was performed for evaluating the data. In histological assessment, laser group showed significantly more osteogenesis and angiogenesis (p<0.05). The findings of the present study show that GaAlAs irradiation together with the application of DFDBA led to improved healing, more angiogenesis, and osteogenesis in bone lesions of rabbit.