The lethal concentration (LC50) of Zataria multiflora essential oil in fries of rainbow trout (Oncorhynchus mykiss)

This research was carried out to determine LC50 for Zataria multiflora essential oil in rainbow trout (Oncorhynchus mykiss) fries at 96 hours. In the process, we used the static O.E.C.D method for 180 rainbow trout fries weighing 5±1 grams in five treatments and one control with three replicates. During the experiments, the mean (±SD) of water temperature was kept at 16±1 C, dissolved oxygen (DO) was 7±0.2mg/l and pH was 8±0.4. We used 5- 25ppm of the essential oil and the results were analyzed using probit analysis procedure. The LC50 96h of Zataria multiflora essential oil was determined as 13.6ppm for rainbow trout (Oncorhynchus mykiss) fry. Based on the results, the useful maximum concentration of Zataria multiflora essential oil was less than 10ppm in rainbow trout fry. The results indicated a narrow therapeutic index for Zataria multiflore essential oil.