فهرست مطالب

  • Volume:7 Issue: 2, 2019
  • تاریخ انتشار: 1398/01/12
  • تعداد عناوین: 9
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  • Babak Abdolkarimi *, Javad Javaheri, Iraj Sedighi, Mahshid Garmsiri, Saman Sedighi Pages 105-112

    Asthma is the most common cause of recurrent wheezing, cough and dyspnea in childhood. Delay in diagnosis and lack of appropriate treatment can cause sustained changes which decrease respiratory capacity of children in future. Therefore, identifying the symptoms relating to asthma is the key for timely diagnosis and treatment of this disease. This study was done to investigate asthma incidence in khomein and compare it to other cities of Iran susceptible to asthma, including Tehran. The present study was a descriptive cross-sectional one based on standard questionnaire of International Study of Asthma and Allergies in Childhood (ISAAC). For conducting this study, 1621 students of first grade in some primary schools and junior high schools of khomein were selected through nonrandom sampling and census and were evaluated during 2015-2016. Both groups were studied and evaluated in terms of being affected by asthma and its severity (current and previous wheezing ,night-time cough, talking limitation due to coughing or wheezing, exercise-induced wheezing, nocturnal awaking due to cough). Results were analyzed through the chi-square test. The asthma diagnosed in Khomein students by the physician during the study was 3.5% while 73.2% of the children under study had at least one asthma symptom. One eighth of the students under study had severe asthma. Comparing with polluted Tehran and northern cities of Iran, Asthma symptoms incidence was higher in khomein where the humidity and industrial pollution is less than in those cities and the asthma cases diagnosed by physicians were much fewer than expected asthma incidence.

    Keywords: Asthma, Wheezing, Hypersensitivity, Iran
  • N Pradeep *, Lekshmi Edison Pages 113-122
    Actinomycetes are inexhaustible producers of commercially valuable metabolites, are continually screened for beneficial compounds. The taxonomic and phylogenetic study of novel actinomycetes strains are mostly based on conventional methods and primary DNA structure of 16s rRNA. Although 16s rRNA sequence is well accepted in phylogeny studies, its secondary structures have not been widely used. RNA Secondary structure especially contains molecular morphometric information which is not found in primary sequences. In this scenario, a comparative study based on both conventional and molecular morphometric approach was conducted for identifying the phylogeny of Western Ghats actinomycete isolate TBG-CH22. The strain was found to a potent producer of beta-glucanase enzymes on the basis of preliminary screening. The taxonomic identification was done with conventional methods, sequence analysis, and molecular morphometric properties of 16s rRNA secondary structure. On account of conventional and primary sequence analysis, the isolate TBG-CH22 was identified as Streptomyces species and delineated evolutionary relationship of the strain within taxa. Predicted evolutionarily conserved secondary structure of 16s rRNAs with minimum free energy levels confirmed the closest phylogenetic neighbour of strain.
    Keywords: betaglucanase, 16s rRNA gene, secondary structure
  • Marzieh Toupal Poudineh * Pages 123-128
    Objective
    Pomegranate juice is a fruit native to Iran because of attractive color, smell and value of mineral water is popular fruits in the world. Pomegranate juice has more nutritional value is due to a combination of anthocyanins that reduce the risk of diseases such as cancer. Processes Feta membrane such as microfiltration 1 and 2 are used to clarify beer industry.
    Methods
    The advantage of this method compared to traditional methods require less labor, higher yields and the process is low. Making a mathematical model or artificial intelligence to predict the juice clarification process in membrane systems is a valuable tool in the field of membrane science and technology.
    Results
    These models play an important role in the simulation and optimization of transparency in membrane systems in order to achieve an economic and efficient design Play. Many of the older models Polar models, the osmotic pressure and boundary layer model have been used to simulate the performance tuning 1 fruit juices.
    Conclusion
    In this study, we tried to take advantage of four regression system, fuzzy inference, neural networks, and fuzzy-neural adaptive method for predicting the flow of water permeate the membrane pomegranate transparency in the system assessed
    Keywords: Pomegranate juice, Processes membrane, Mathematical models
  • Emmanuel Okrikata *, Anuoluwa Yusuf Pages 129-141
    A field survey was conducted in Wukari, Taraba State to assess the diversity and abundance of insect species in selected habitats (residential, open field made up of grassland and an agro ecosystem). Insects recovered were wet preserved in 70% ethanol except butterflies and moths. A total of 4,501 insects spread across 9 orders, 34 families and 77 species were recovered. The most dominant order was Coleoptera with a relative abundance of (44.41%) and, the least was Orthoptera (0.84%). The most dominant insect species are Heteronychus mossambicus (11.44%) followed by Termes sp. (7.77%) and, Goryphus sp. (7.71%). Chlaenius decipiens, Cheilomenes sulphurea, Copris sp., Cicindela sp., Pseudantheraea sp., Derobranchus geminatus, Glaurocara townsendi, Camponotus perrisi, and Gryllus bimaculatus were the rare species with relative abundance of 0.02%. Species richness is based on number of individual insects measured. The highest species diversity was observed in the order Coleoptera (Shannon H’ =2.547) while, Isoptera was the least (H’ = 0.00). However, the highest species evenness was observed in the order Isoptera (E’ = 1.00). Fisher-alpha (α) index of diversity shows that the agro ecosystem has the highest index of diversity (α = 14.24) while, the residential area had the least (α = 11.9). This study therefore, brings to the fore the diversity and abundance of insects in Wukari and underscores the need for a more intensive study and for sustainable actions to be taken in conserving beneficial rare species while, managing the abundant pestiferous ones.
    Keywords: Diversity, Fisher-alpha (α), Insects, Jaccard’s similarity index, Shannon index (H’)
  • Armin Gharibi *, Roudabeh Behzadi Andouhjerdi Pages 142-150
    Background
    Cytokeratins (CK) are the largest subgroup of interstitial proteins, and their expression changes during the development of cancer. Cytokeratin (CK-18) is one of the major proteins of the epithelial cell skeleton. The expression potential of the CK-18 gene was studied in this research as a molecular biomarker for the diagnosis of breast cancer in the circulatory system using the Real-Time PCR technique.
    Methods
    Blood samples of breast cancer patients and healthy individuals (as the control group) were collected from the Cancer Institute of Imam Khomeini Hospital, Tehran, Iran and their RNA was extracted. In the next step, the cDNA molecule was synthesized using a reverse transcriptase enzyme (RT), and gene-specific primers were designed and synthesized. Then, the expression of CK-18 tumor marker was evaluated by the real-time PCR technique; finally, the data obtained from the cancer samples and the control group was analyzed by the SPSS software.
    Results
    CK-18 expression was observed and measured in the patients, serum .In addition, according to the disease grade, the CK-18 expression was different in the patients, serum.
    Conclusions
    The findings of this research can be used in future studies exploring the mechanism of action of this gene as a suitable target for the treatment of breast cancer.
    Keywords: Keywords Circulating tumor cells (CTC), Breast cancer, CK-18, tumor marker, Real-time PCR
  • Reza Yavari, Saeed Zaker Bostanabad, Armin Gharibi * Pages 151-159
    Introduction Colorectal cancer is the second and third most common cancer in women and men respectively. Early diagnosis of illness will reduce pain and costs for patients. In this study, the expression of fgf19 gene in different individuals with colorectal cancer, and also the relationship between the expression of this gene and the degree and stage of cancer cells, will be evaluated.Methods A total of 60 samples were collected by a surgeon from cancerous tissue and healthy marginal tissue of patients with colorectal cancer. Extraction of RNA was accomplished by a Trizol solution. In the next step, cDNA molecule was synthesized using reverse transcriptase enzyme (RT), and gene-specific primers were designed and synthesized. Then the expression of FGF19 gene was evaluated by Real-time PCR technique. Finally, the data obtained from cancerous tissue and healthy marginal tissue were analyzed by spss software.Result The expression of fgf19 in tumor tissue and healthy marginal tissue was measured quantitatively, which increased by 56.7 percent.Conclusion The results of this study indicate that fgf19 as a molecular biomarker plays a significant role in the progression of cancer, and therefore can be considered as a factor in the screening, early detection, prognosis and predictor of the tumor.
    Keywords: Colorectal cancer, FGF19, Tumor Tissue, RT-q PCR, tumor marker
  • Soheil Afshari Behbahanizadeh, Samad Farashi Bonab * Pages 160-172
    Breast cancer is a public health concern among the women. iNOS is stated by the effect of various inflammatory factors and is thus called inducible NOS. Investigating iNOS expression is a potent tool for understanding effective molecular parameters in tissue and cellular responses to external factors. In this research study, iNOS expression in mice with breast cancer was investigated and the effects of various doses of indomethacin on the iNOS gene expression in breast tumors was evaluated.4T1 cells were grown in RPMI 1640 Medium. 200 µl of cell suspension (107 cells)were injected into the mice right flank, subcutaneously. 35 Balb/C albino mice divided into five groups each group consist of 7 mice. First group was healthy control group, second group was tumor control, and the 3rd, 4th and 5th groups were treated by 25μg, 50μg and 100μg of indomethacin, respectively. The rate of breast tumor growth was measured in treated groups for two months. The levels of iNOS gene expression was determined using real time RT-PCR technique.Our results demonstrated, iNOS expression in tumor tissue increased with the growth of breast cancer cells. Furthermore, Administration of 25μg and 100μg indomethacin neither had significant effect on breast tumor growth nor effect on iNOS gene expression. While 50μg indomethacin decreased the tumor growth (P>0.05) and decreased iNOS gene expression. Further studies such as evaluating the effects of indomethacin on the other anti-tumor immunosuppressing factor and additional tests western blot, flow cytometry and immunohistochemistry recommended in order to obtain more practical findings.
    Keywords: Indomethacin, Gene expression, iNOS, breast tumor
  • Alagbe John *, R.A. Oluwafemi Pages 173-183
    This experiment was carried to determine the growth performance of weaner rabbits fed Morinda citrifolia (Noni) and Moringa olifera leaf meal mixture (MCML) as partial replacement of Soybean meal (SBM). Fifty (50), 7-8 weeks bucks cross breed rabbits (Chinchilla × New Zealand White) with an average weight of 620g and 625g were allotted into five (5) dietary treatments of ten (10) rabbits per group and were individually caged in an all –wired metabolic cages. SBM was replaced by MCML at levels of 0%, 3%, 6%, 9% and 12% respectively and the experiment lasted for 98 days. Clean feed and water were provided ad libitum, experimental parameters covered feed intake, feed conversion ratio, daily water intake and mortality. The results of this experiment showed that there were no significant differences (p>0.05) in the final weight gain, feed intake, feed conversion ratio and daily water intake across the treatment, diet containing 3% MCML had the highest weight gain of 1157.0g, while rabbits fed 0% MCML had the lowest weight gain of 1084.0g. Significant differences (p
    Keywords: Weaner rabbits, Performance, Soybean meal, Moringa olifera, Mortality
  • Tawfeeq Auqbi *, Yossra K. Al, Robaiaay, Tawfeeq F.R. AL, Auqbi Pages 184-190
    Objectives
    There is a common belief among Muslims, to consume dates in odd number rather than even, based on cultural and religious claims; although nobody knows its effect on blood glucose level or other metabolic effects in the body.The aim of our study is to illuminate the changes that affect the 2-hrs postprandial serum glucose level after the consumption of an odd number of dates among healthy young persons and even number.
    Method
    Intervention study was used to compare 2 groups, Fasting glucose levels was measured for all participants; group A (42 participants), who consumed even number of dates all at Tamer-stage, (net weight about 50 grams); and group B, (42 participants), presumed to consume an odd number of dates (net weight about 40 grams). Two-hrs postprandial blood glucose levels were measured altogether. Blood glucose levels were determined and compared as a means. Statistical analyses were performed using SPSS version 22.
    Result
    The fasting serum glucose of group A and B participants were 91.65±13.61 and 93.69±8.79 mg/dl, respectively, with an insignificant statistical difference between groups (P= 0.42327). Subsequently, the 2hrs Postprandial Glucose measurements of group A and B were 99.58±19.56 and 100.33±12.204 respectively; indicated an insignificant statistical difference between groups, (P=0.83553). All the participants were remaining within normal ranges of serum glucose level.
    Conclusion
    There is no difference between ingestion of the odd and even number of dates fleshes from the glycemic point of view on the glucose level in fasting and postprandial states.
    Keywords: eating dates, odd, even, glucose level