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Food Quality and Hazards Control - Volume:8 Issue: 3, Sep 2021

Journal of Food Quality and Hazards Control
Volume:8 Issue: 3, Sep 2021

  • تاریخ انتشار: 1400/08/04
  • تعداد عناوین: 8
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  • S. Aslam, N. Khalid* Pages 92-93
  • M. Fatahi-Bafghi, H. Zandi* Pages 94-95
  • M.A.R. Khan, M.S. Islam, M.S.A. Mamun* Pages 96-103
    Background

    Among the local fish species in Bangladesh, Labeo rohita and Barbonymus gonionotus are two popular fish species. The present work focused on proximate compositions (moisture, protein, fat, and ash) and risk assessment of lead in two common fishes (L. rohita and B. gonionotus) collected from river and farms of Bagerhat, Bangladesh. Furthermore, effect of freezing condition on fish lipid was studied. 

    Methods

    Proximate compositions of the fish samples were determined according to conventional methods. The lead contents were analyzed by wet digestion method using an atomic absorption spectrophotometer. The health risks models such as Estimated Daily Intake (EDI), Target Hazard Quotient (THQ), Hazard Index (HI), and Target Cancer Risk (TCR) were conducted for health risk assessment of the local people. Data were analyzed using SPSS software (version 16.0).  

    Results

    A positive correlation was found between moisture-protein and lipid-ash of the L. rohita fish. Besides, a positive relation was observed in ash-moisture, and lipid-protein for B. gonionotus. The lead content in river L. rohita, farm L. rohita, river B. gonionotus, and farm B. gonionotus was observed as 9.00, 9.20, 8.29, and 6.39 mg/kg, respectively. The lead content found in the fish species were above the permissible limit. The TCR revealed the carcinogenic effects to the local people due to exposure of lead.

    Conclusion

    This study reflects some data about proximate values of L. rohita and B. gonionotus fishes. The concentrations of lead in the fish samples were above the safe limits, and may have carcinogenic effects of the local consumers in Bagerhat, Bangladesh.

    Keywords: Nutritive Value, Lead, Metals, Heavy, Risk Assessment, Fishes, Bangladesh
  • N.K. Kortei*, P.Y. Atsugah, E. Letsyo, A. Abaka-Yawson, A.A. Boakye, C.O. Tettey, E.K. Essuman Pages 104-111
    Background

    Natural Food Toxins (NFTs) are poisonous substances produced by the biological processes of living organisms. This study aimed to evaluate the knowledge and attitude of tertiary students of the Volta Region of Ghana about NFTs.

    Methods

    During March to June 2020, semi-structured questionnaires were used to examine knowledge and attitudes about NFTs among 252 tertiary students from various institutions of the Volta Region of Ghana. Data were statistically analyzed using IBM SPSS Statistics version 20.0.

    Results

    A majority (90.48%) of students were aware of the NFTs. The male students showed significantly (p<0.05) greater knowledge than the females. Totally, 190 out of 252 (75.40%) respondents had prior-to-the-study knowledge about NFTs. Also, 245 out of 252 (97.22%) respondents desired to learn more about NFTs.

    Conclusion

    From this study, it was observed that tertiary students from the various institutions in the Volta Region of Ghana had enough knowledge and attitude about NFTs; but there are still slight deficits in the knowledge of students about types of NFTs. Hence, there is a need for more sensitization of the subject among the students and throughout the general public.


    Keywords: Knowledge, Attitude, Surveys andQuestionnaires, Food, Toxicology, Ghana
  • S.S. Nyirenda*, K.M. Bukowa, W.R. Banda, J. Mbewe, F. Hamankolo, F. Banda, G. Kwenda, M. Mumba, E. Zulu Pages 112-118
    Background

    Consumption of raw or unhygienically prepared vegetables is a potential source of parasitic infection. This study aimed to establish the prevalence and types of intestinal parasites on the freshly sold vegetables at the market.

    Methods

    Totally, 95 vegetable samples were randomly procured from vendors at Lusaka City market (Zambia). The samples were examined for parasitic contamination using floatation and sedimentation methods. Data were analyzed using Epi Info version 7.2.4.0.

    Results

    The overall parasitic contamination of fresh vegetables was 35.8% (34 out of 95). The highest parasitic contamination rate was found in Chinese cabbage with 7.4% (7 out of 95), followed by rape with 6.3% (6 out of 95), chilli with 5.3% (5 out of 95), pigweed with 5.3% (5 out of 95), pumpkin leaves with 5.3% (5 out of 95), tomatoes with 4.2% (4 out of 95), and cabbage with 2.1% (2 out of 95). There was a significant (p=0.001) statistically difference between the type of vegetables and the presence of the parasites. The identified helminths had different prevalence rates, including Taenia spp. with 9.5%, Trichuris trichuria with 5.3%, Ascaris lumbricoides with 3.2%, Clonorchis sinensis with 2.1%, Diphyllobothrium latum with 2.1%, Paragonimus westermani with 2.1%, and Strongyloides stercoralis with 2.1%. Also, Giardia lamblia with 24.2% and Balantidium coli with 4.2% were the protozoan parasites identified.

    Conclusion

    This study showed that some vegetables sold at the Lusaka City market (Zambia) were a potential source of parasitic infections for local consumers. G. lamblia was the most prevalent parasite found in the vegetable samples.


    Keywords: Vegetables, Parasites, Parasitic Diseases, Giardia lamblia, Risk Factors, Zambia
  • M.L. Baraquet, O.F. Camiletti, C.I. Moretti, L.E. Rodríguez, C. Vázquez*, M.G. Oberto Pages 119-124
    Background

    The changes and the availability of processed foods have increased the demand for ready-to-eat foods, such as Minimally Processed Vegetables (MPVs). The purpose of this work was to evaluate the microbiological status and quality traits of the MPVs obtained from retail outlets of Córdoba, Argentina.

    Methods

    Totally, 60 MPVs of 12 brands (30 single-ingredient salads and 30 mixed salad trays) were randomly sampled from different retail outlets of Córdoba, Argentina. The samples were analyzed according to international standards for Total Coliforms (TC), Fecal Coliforms (FC), Escherichia coli, Salmonella spp., Staphylococcus aureus, and Yeast and Molds (YM). The quality traits (respect to physical and sensory properties) of the MPVs were evaluated. Statistical analyses were performed with InfoStat.

    Results

    All 60 MPV samples were positive for TC, ranging from 1.32 to 3.38 log Most Probable Number (MPN)/g. FC counts ranged from 0.30 to 1.95 log MPN/g. Out of 60 samples, 15 (25%) were positive for E. coli. Three brands of mixed trays were positive for S. aureus. Regarding sensory characteristics, the parameters such as freshness, turgid, normal color, decay, and dehydration were compliance in 42 out of 60 (70%) samples.

    Conclusion

    Some MPVs sampled from Córdoba, Argentina showed low microbiological quality and imperfect quality traits.


    Keywords: Vegetables, Escherichia coli, Staphylococcus aureus, Salmonella, Fungi, Argentina
  • M.M. Soltan Dallal*, H. Abdolmaleki, R. Ramazanzadeh, R. Mazaheri Nezhad Fard Pages 125-130
    Background

    When animal manures are used, food products may include pathogenic bacteria, especially Escherichia coli. The major aim of the current study was to investigate TEM (blaTEM) and SHV beta-lactamase (blaSHV) genes in E. coli isolated from strawberry samples in Sanandaj, Iran.

    Methods

    In this study, 150 strawberry samples were collected from farms (traditional), greenhouses, and packages in Sanandaj, Iran. E. coli contamination was done using routine culture methods. Then, isolates were investigated for Extended-Spectrum Beta-Lactamase (ESBL) production and blaTEM or blaSHV genes using phenotypic and genotypic methods, respectively.

    Results

    The most susceptibility and resistance of E. coli to antibiotics were related to chloramphenicol and trimethoprim/sulfamethoxazole, respectively. Out of 21 isolates of E. coli, eight were resistant to ceftazidime and cefotaxime; from which, six isolates were ESBL-producer. Furthermore, Polymerase Chain Reaction (PCR) analysis of six ESBL- producing E. coli isolates showed that four isolates included blaTEM gene, while no isolates included blaSHV gene.

    Conclusion

    In this study, multiple antibiotic resistance patterns were seen in E. coli isolates, especially ESBL patterns in E. coli isolated from strawberries produced in Iran. 


    Keywords: Escherichia coli, beta-Lactamases, Drug Resistance, Microbial, Polymerase Chain Reaction, Fragaria, Iran
  • H.H. Makumbe, F.T. Tabit*, B.C. Dlamini Pages 131-139
    Background

    Food contamination with Listeria spp. can occur at all stages of the food chain. The aim of this research was to investigate the prevalence, molecular identification, antimicrobial resistance, and disinfectant susceptibility of Listeria innocua isolated from Ready-To-Eat (RTE) foods sold in Johannesburg, South Africa.

    Methods

    Eighty RTE foods were collected from Johannesburg, South Africa. The 16S rRNA region of L. innocua isolates was amplified, sequenced, and identified using Basic Alignment Search Tool (BLAST). The antimicrobial resistance and disinfectant susceptibility (against four commercial disinfectants) of the isolates were evaluated using disk diffusion and microdilution assays. Data were statistically analyzed using SPSS v. 23.0.

    Results

    Listeria strains revealed a high 16S rRNA gene sequence analogy to L. innocua of between 98-99%. The overall prevalence of L. innocua was 21.3% (17 out of 80) in the RTE food samples. Most isolates were susceptible to the studied commercial disinfectants. All the L. innocua isolates from food sources were found to be resistant to ampicillin and cephalothin, while 83 and 74% of isolates were resistant to colistin sulphate and sulphatriad.

    Conclusion

    Prevalence of L. innocua was considerable in the RTE food samples sold in Johannesburg, South Africa. The L. innocua isolates showed high antibiotic resistance against ampicillin, cephalothin, colistin sulphate, and sulphatriad.


    Keywords: Listeria innocua, Drug Resistance, Microbial, Disinfectants, Polymerase Chain Reaction, Fast Foods, South Africa