مجله دانشکده پزشکی اصفهان
پیاپی 654 (هفته دوم اسفند 1400)

Mesenchymal stem cells are able to differentiate into osteoblasts, which is forced by several growth factors. Similar to growth factors, plant metabolites have been shown to be able to support stem cell differentiation. The aim of this study was to assess the synergistic effects of resveratrol-poly(lactic-co-glycolic acid) (PLGA) and curcumin-PLGA nanoparticles on directing adipose-derived mesenchymal stem cells towards osteoblastic differentiation.

Human mesenchymal stem cells derived from the adipose tissue, after being characterized by flow cytometry, were simultaneously treated with 10 μM concentrations of each of PLGA-resveratrol and PLGA-curcumin nanoparticles. The cytotoxic effects of resveratrol and curcumin were evaluated by the MTT assay, and their differentiating potentials were evaluated by Alizarin red staining.

According to MTT results, neither resveratrol-PLGA nor curcumin-PLGA nanoparticles had no significant toxic effects on the target cells after 24 hours. At the 20 and 10 μM concentrations of resveratrol-PLGA and curcumin-PLGA nanoparticles, respectively, a significant increase was observed in the proliferation of human mesenchymal stem cells. Alizarin red staining showed that treatment with resveratrol-PLGA and curcumin-PLGA nanoparticles increased the deposition of mineral ions in the extracellular matrix, an indicator of the onset of the cellular differentiation process.

The results of this study showed that the simultaneous use of bioactive compounds such as resveratrol-PLGA and curcumin-PLGA nanoparticles can direct stem cells towards osteoblastic differentiation. The simultaneous use of resveratrol-PLGA and curcumin-PLGA nanoparticles increases their efficiency at lower concentrations due to their synergistic effects.

Migraine headaches are one of the psychosomatic disorders that severely impair patients' quality of life and general functioning by mediating psychological problems. The aim of this study was to investigate the mediating role of pain anxiety in the relationship between psychosomatic components on quality of life in these patients.

In this cross-sectional study, using available sampling method, 250 patients with migraine were selected based on inclusion and exclusion criteria among people with migraine referred to the Pain and Neurology Clinic affiliated to Isfahan Medical Sciences, Isfahan, Iran, in year 2020. In the appropriate psychological situation, after justifying the goals of the research, the World Health Organization Quality of Life Questionnaire (WHOQOL-BREF), McCracken Pain Anxiety Questionnaire (PASS-20) and also the Diagnostic Criteria for Psychosomatic Research Questionnaire (DCPR) were completed.

There was a negative correlation between the components of psychosomatic and pain anxiety with the dimensions of quality of life. Moreover, there was an indirect relation between the components of psychosomatic with the dimensions of quality of life, a direct relation between pain anxiety and the dimensions of quality of life. Besides, the proposed model of path analysis of variables had a good fit.

The findings of this study explain the contribution of some underlying psychological factors in the occurrence and persistence of headache. Moreover, this study suggests the development of psychological intervention packages based on an explanatory model to improve the quality of life of patients with migraine.

Laparoscopic cholecystectomy is the standard treatment for gallbladder diseases. Hepatic artery pseudoaneurysm is a rare complication of bile duct injury. Rupture is accompanied by abdominal pain, intraperitoneal bleeding, jaundice, and gastrointestinal bleeding (GIB). In this study, we introduce a patient who underwent laparoscopic cholecystectomy. During the laparoscopic operation the patient needed open surgery due to bleeding; and a month later, the patient presented with massive upper gastrointestinal bleeding.

The patient was a 43-year-old woman with a history of laparoscopic cholecystectomy one month before. She presented with complaints of abdominal pain and hematemesis. Gastrointestinal endoscopy showed duodenal bulb erosion, and computed tomography (CT) scan showed evidence in favor of hepatic artery pseudoaneurysm. The patient underwent emergency laparotomy due to hemorrhagic shock. Hematoma and pseudoaneurysm of the right hepatic artery from superior mesenteric artery were seen in the exploration. The exploration of subhepatic hematoma, hepatic artery repair, and abdominal cavity drainage was done. A few days after the operation, the patient was discharged in good general condition, stable vital sign, normal laboratory tests, and PO tolerance. There was no complication in one month follow-up.

Vascular injuries are rare complications in patients undergoing cholecystectomy, and its manifestation as UGIB is very rare. Due to its high morbidity and mortality, it should be evaluated and treated as soon as possible.