مجله علوم پزشکی پارس
سال بیست و یکم شماره 2 (پیاپی 64، تابستان 1402)

 The nature of septorhinoplasty surgery and hemodynamic instability in patients causes intraoperative bleeding, which affects the quality of the surgical field of view, intraoperative and postoperative complications, and surgical outcome.

This double-blind randomized clinical trial study was conducted on 60 patients aged 18 to 44 who were candidates for septoranioplasty surgery. Patients were randomly divided into two equal groups. Group 1: received bisoprolol 2.5 mg and group 2 received placebo as a control group. Hemodynamic symptoms will be measured before induction, after induction, 1 minute after induction, 5 minutes after induction, 30, 60, 90 and 120 minutes during operation, entering recovery and exiting recovery. The amount of bleeding during the operation was estimated based on the amount of bleeding in the suction.

In the times before and after induction, 1 minute, 5, 15, 30, 60, 90, 120, in recovery and outside of recovery, there was a significant difference between the two groups in the average systolic and diastolic blood pressure and blood pressure. There was no mean arterial (P<0.05). While the heart rate in these minutes was reported to be significantly lower in the bisoprolol group than in the control group (P<0.05).

The results of this study showed that the use of a dose of 2.5 mg of bisoprolol before the operation can significantly reduce the heart rate, but it does not make a significant difference in the hemodynamic changes and the bleeding rate of patients in septorhinoplasty surgery.

This study investigated the effects of hydoalcoholic Aloe vera extract on fetal cerebellar tissue in diabetic pregnant rats.

Forty eight adult pregnant Wistar rats were divided into 6 groups of 8 including control(C), receiving 400mg/kg Aloe vera extract(CA), diabetic(D), diabetic receiving 20IU/kg insulin(DI), diabetic receiving 400mg/kg Aloe vera extract(DA) and diabetic receiving 20 IU/kg insulin and 400mg/kg Aloe vera extract(DAI). The rats received insulin subcutaneously and the Aloe vera extract through gavage. Blood glucose and body weight were recorded on days 1,7,14 and 18 of gestation. On day 18, the weight of each rat and the number and gender of its fetuses were recorded. Fetal brains were removed and tissue sections of their cerebellum were prepared. The results were analyzed through ANOVA and at the significant level of p≤0.05.

Blood glucose in diabetic groups was significantly higher than C and CA groups. Weight of pregnant rats on days 7,14, and 18 significantly decreased in the D,DI, and DAI compared to the C and CA groups. The number of newborns significantly decreased and the neonatal weight increased in all diabetic groups compared to the C and CA groups. Compared to the C group, the thickness and number of gray and white matter cell bodies and thickness and number of cells in the molecular layer,Purkinje and granular cells significantly decreased in the D group compared to the C group.

Aloe vera extract was able to prevent the effects of excessive production of oxidants in diabetes.

Mismatch blood group antigens between blood donor and recipient increases the risk of alloimmunization. The aim of this study was to detect the frequency of unwanted alloantibodies against blood group antigens in beta-thalassemia patients receiving continuous blood transfusions in Jahrom City.

In this descriptive study, a total of 79 patients with beta-thalassemia were enrolled. Alloantibodies were evaluated using antibody screening and identification (panel test) methods. Statistical analysis was performed with SPSS version 23. All data were statistically analyzed using descriptive statistics methods and Fisher's exact test.

In this study, 10 patients (12.65%) had alloantibodies against red blood cells (RBCs). The most commonly detected alloantibodies in patients were anti-K (50%), anti-E (20%), and anti-c (20%). The rate of alloimmunization was significantly lower in transfused patients with leukoreduced RBCs compared to those transfused with non-leukoreduced RBCs (P<0.001). Patients with O+ blood group produced the most alloantibody, which was not statistically significant (p=0.61). There was no statistically significant correlation between the prevalence of alloantibody with the sex and age variables (p>0.05).

Based on the results, the most commonly detected alloantibodies were against Kell and Rh (E, c) blood group systems, which can be useful in deciding to store blood components with rare blood groups.

The management of Candida-related infectious diseases and the recognition of drug susceptibility patterns in each region depend on local epidemiological studies and population structure of each region. The present study was carried out with the aim of identifying the common species associated with VVC and determining their antifungal susceptibility profile.

In this study, 140 clinical isolates of Candida species were collected from people with vaginitis referred to a number of health centers in Zanjan, Iran. Antifungal susceptibility tests for fluconazole, itraconazole and ketoconazole was performed by broth microdilution method, and the results were statistically analyzed using SPSS software.

Overall 41 Candida species were isolated from vaginal discharge samples. C. glabrata was the most frequent with 23 isolates (56.1%). Candida isolates showed high resistance to azoles, so that 11 isolates (26.8%) were resistant to fluconazole. Also, the resistance to itraconazole and ketoconazole was found to be 58.5% and 65.9%, respectively.

In this study C. glabrata had the highest prevalence and it shows the change in the pattern of Candida infections towards non-albicans species. The occurrence of high drug resistance to azoles is one of the problems facing vaginal candidiasis, which leads to recurrent and treatment-resistant infections.

APC gene in ctDNA and miR-21-5p have been proposed as potential biomarkers for cancer diagnosis. In this above study, a biosensor based on multi-walled carbon nanotube (MWCNT) and DNA probe with fluorophore FAM (6-carboxyfluorescein) to detect APC gene in ctDNA, and DNA probe with fluorophore ROX (5-carboxy-x-rhodamine ) to detect miR-21-5p, was designed to identify patients with colorectal cancer (CRC).This method is based on the adsorption and stabilization of single-stranded DNA (ssDNA) labeled with FAM and ROX on MWCNT, which leads to the quenching of the fluorescence emission of FAM and ROX. By adding their complementary DNA (cDNA), which led to the release of single-stranded DNA (ssDNA) probes from the MWCNT surface and a double-stranded DNA (dsDNA) was formed, which led to the return of FAM and ROX fluorescence emission. In this study, the sensitivity of FAM fluorophore was 50 nM (50 nM) and the sensitivity of ROX fluorophore was 25 nM (25 nM). The response time in the presence of the target strand is 12 minutes. The LOD and LOQ of DNA biosensor individually were 1.12 nM and 3.2 μM, respectively. The results of this study showed that the biosensor based on carbon nanotubes and DNA probe, in addition to existing methods, can be used as a high-sensitivity method for early detection of CRC.

Colon cancer is caused by abnormal growth of cells that can invade (Metastasis) or multiply in other tissues in the body. Colorectal cancer is the third most common cancer in the world and its mortality rate is almost 50% of patients. Research has shown that LncRNAs play an important role in tumorigenesis, angiogenesis, proliferation, migration, apoptosis and differentiation. Based on the above, the present study investigates the expression of LncRNAHPYR1 in patients with colorectal cancer.Materials &

The present study was performed by sampling 20 patients with colorectal cancer and 20 healthy tissues. Then RNA extraction was performed by Trizol kit and cDNA synthesis was performed by Takara kit and finally the expression of the relevant gene was performed by Real Time RT PCR technique. The relative expression level of LncRNAHPYR1 was calculated and finally the results were analyzed by GraphPad Prism software.

The results showed that the expression of HPYR1 lncRNA in colorectal cancer has a significant increase compared to the control group (p=0.0116) and also the expression of the gene is related to metastasis (p=0.002). On the other hand, the relative expression of HPYR1 lncRNA in the tumor of people with stage 1 and 2 and in the tumor of people with different size tumors did not show a significant increase (p=0.56) and (p=0.8270), but the relative expression of lncRNA HPYR1 in the tumor of people with grades 2 had an increase in expression compared to grade 1 and it was statistically significant.

Laryngeal spasm is the narrowing or complete closure of the glottis due to the reflex contraction of the laryngeal muscles. The present study reports a case of upper airway obstruction following long-term use of a laryngeal mask in surgery.

A 45-year-old female patient who referred to a urologist due to urological problems with complaints of urinary bleeding and pain in the urogenital area. Based on trusting the surgeon's words about the shortness of the surgery, a laryngeal mask (LMA) was used for the patient, and propofol was used to anesthetize the patient during the operation. The patient's surgery gradually took longer due to suspicion of malignancy in the urinary tract and bladder, and the surgeon extended the surgery almost every 15 minutes. Finally, the surgery was completed after nearly three hours, and despite the fact that the patient had not taken any special medicine except propofol an hour before the end of the operation, but when he woke up, he had difficulty breathing. After about 30 minutes passed and the conditions were suitable, we extubated the patient with special precautions and transferred him to the intensive care unit for recovery and for further assurance.

Based on the results of the present study, long-term use of laryngeal mask is associated with the occurrence of laryngospasm after surgery, which can be considered as a result of long-term stimulation of the upper airway by the laryngeal mask.