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Food Quality and Hazards Control - Volume:11 Issue: 1, Mar 2024

Journal of Food Quality and Hazards Control
Volume:11 Issue: 1, Mar 2024

  • تاریخ انتشار: 1403/01/07
  • تعداد عناوین: 8
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  • V. Toupchi-Khosroshahi, E. Mehrabi Nasab* Pages 2-3
  • G. Aboagye*, P.G. Akpaloo, T.A. Avle Pages 4-12
    Background

    Water quality and safety are fundamental to human development and well-being. Therefore, the purpose of this study was to determine the bacteriological and mineral content of water in Ho, the capital city of the Volta Region of Ghana.

    Methods

    Sachet, bottled, and tap water were sampled from January to February in 2019 due to the high rate of consumption and their presumed quality which were taken at random from five different locations throughout the municipality. Water quality assessment protocols were utilized to ascertain the bacteriological as well as mineral contents of the samples, whilst ANOVA was used to determine statistical difference and significance at p<0.05.

    Results

    The maximum Heterotrophic Plate Count for tap water was 9.95±0.64×105 Colony Forming Unit (CFU)/ml, for sachet water was 7.46±0.09×106 CFU/ml, and for bottled water was 1.10±0.56×105 CFU/ml, all obtained on nutrient agar. For MacConkey agar, maximum growth was 2.94±0.03×106, 9.42±1.67×106, and 2.31±0.77×105 CFU/ml for tap, sachet, and bottled water, respectively. The Xylose Lysine Deoxycholate Agar indicated maximum growth of 1.84±0.34×103, 5.72±0.06×106, and 5.50±2.12×104 CFU/ml for tap, sachet, and bottled water, respectively.
    The physical parameters such as pH, turbidity, color, and conductivity were within the recommended limits set by the Ghana Standards Authority. However, tap water recorded the highest turbidity, bottled water recorded the highest and least pH and turbidity, respectively. Moreover, the mineral analysis revealed high levels of phosphate (PO₄³⁻), chloride (Cl-), and sodium (Na) in bottled water, and total iron (Fe) was relatively high in several tap and sachet water samples, the latter item also recorded the highest for ammonia (NH3).

    Conclusions

    Overall, the tap, sachet, and bottled water samples exhibited varied levels of microbial, and mineral contents whilst the physical parameters were relatively within the recommended levels. The sachet and tap water were the least wholesome in comparison with the bottled water samples.

    Keywords: Drinking Water, Minerals, Colony Count, Microbial, Bacterial Load, Ghana
  • P.O. Oleghe, F.C. Akharaiyi*, C.B. Ehis-Eriakha Pages 13-24
    Background

    The exorbitant cost of wheat-based foods in non-wheat growing countries has necessitated looking for more enriched and sustainable alternative flour from botanicals that can be mixed and used to produce baked products including biscuits. The study aimed to make biscuits using three different fermented and unfermented mixtures of flour (sweet potato, pigeon pea, and yellow maize).

    Methods

    Starch-rich tubers of yellow-fleshed cultivar of sweet potato (Ipomoea batatas), yellow maize (Zea mays) grains, and pigeon peas (Cajanus cajan) seeds were purchased from food merchants in the Uchi market, located in Auchi area of Edo State, southern Nigeria in June 2022. These botanical samples were taxonomically validated. The samples were divided into two parts fermented and unfermented, prepared and produced into flour forms. The blended raw materials into flour were mixed in order of sweet potato: pigeon pea: maize (composite mix two 60.00:25.47:14.53, composite mix five 67.70:20.00:12.31 and composite mix eight 61.72:25.24:13.04) were selected to produce biscuits while 100% wheat was used as control. Biscuits were produced from the flour using a standard recipe. The obtained results were presented in mean±SD format of interpretations. Analytical significance dissimilarity between the means samples were considered based on one-way analysis of variance (ANOVA) using IBM Statistical software.

    Results

    It revealed that fermentation increased techno-functional properties containing crude fiber (3.464±0.01-3.485±0.01) and ashes (3.688±0.11-3.711±0.11), while reducing fat (17.339±0.03) and moisture contents (3.639±0.05), the control had the highest protein (12.805±0.25) and lowest carbohydrate (55.622±0.12). The fermented biscuits had more flavonoids (23.162±0.36-23.852±0.60), saponins (14.793±0.07-23.495±0.03). Additionally, fermentation enhanced the potassium bioavailability of the products (19.803±0.00-25.264±0.05). There was high free radical scavenging activity and color for all the fermented samples than unfermented and controlled biscuits.

    Conclusion

    According to the research, ternary flour mixes from the botanicals could lead to products with improved nutritional composition, functional properties, and antioxidant attributes to further improve biscuit quality.

    Keywords: Flour, Fermentation, Triticum, Zea mays, Nigeria
  • E.N. Solefack, B. Tiencheu*, R. Zokou, C.T. Tiwo, A.H.F. Kohole, G.B. Teboukeu, S.H. Ndomou, F.D. Tonfack, J.M. Klang, M.H. Womeni Pages 26-38
    Background

    In several cities of Cameroon, the consumption of soy cheese skewers commonly known as “soybean soya” is becoming very common in the streets. Its consumption is increasing thereby encouraging its local production using household methods. Food contamination from processing to consumption is becoming a serious health concern. Therefore, the purpose of this study was to ascertain the microbiological quality of soy cheese skewers sold in some markets and the Critical Control Points (CCPs) in the production process.

    Methods

    Samples were collected in June and August 2022 from different vendors, stored in sterile plastic laminas and placed in a pack containing ice and taken to the laboratory for the determination of the microbiological quality. In order to determine CCPs based on microbiological hazard, samples of slurry, soymilk, fresh soy cheese, stewed soy cheese, and stewed soy cheese skewers were collected from different producers at different production steps and analyzed for microbial contaminations.

    Results

    Results of samples collected from the markets indicated Total Mesophilic Aerobic Bacteria between 4.87 to 7.79 log Colony Forming Unit (CFU)/g, where 86% of the samples were higher than the normal; yeast and mold counts ranged from 2.84 to 5.91 CFU/g; staphylococci, and total coliforms ranged from 0 to 5.17 and 0 to 2.37 log CFU/g, present in 59 and 72% of samples, respectively.

    Conclusion

    Most commercial soy cheese skewer samples showed poor microbiological quality. To reduce these contaminations, preventive measures should be put in place at CCPs (stewing and packaging on skewers) to ensure the safety of food.

    Keywords: Soybean, Cheese, Contamination, Hazard Analysis, Critical Control Points, Cameroon
  • A.O. Oyedeji*, B.A. Odeyemi, L.A. Azeez Pages 39-46
    Background

    The safety of milk is considered as a significant public health consideration and has been a key concern for consumers worldwide. The concentrations of cyromazine and its metabolic product, melamine, and their dietary risk assessment are investigated in this study.

    Methods

    A total of 182 milk samples containing 15 brands were sampled between June and December 2022 from major and retail markets in Nigeria. After a solid-phase extraction procedure, the concentrations of the two compounds were determined using High-Performance Liquid Chromatography coupled with a Diode-Array Detector. Solid-phase extraction was utilized to extract local and imported evaporated and infant milk with CCl3COOH and CH3CN, followed by clean-up with NH4OH in MeOH. The extracts were analyzed with Agilent High-Performance Liquid Chromatography, including a Zorbax Eclipse+C18 column.

    Results

    For both melamine and cyromazine, the Limits of Detection and Limits of Quantification were 1.29-1.48 and 3.94-4.50 µg/kg, respectively. The precision (Relative Standard Deviation<1), recovery (99.5–102.5%), and regression (r2=0.989) were all excellent. Melamine concentration ranged between 57.6±18.9 and 930.3±379.9 µg/kg among the samples, and cyromazine was 57.2±12.3 and 670.9±87.8 µg/kg. Brand 2, imported from Holland, had the highest detection frequency for the two analytes. Disturbing levels of melamine were detected in Brand 2, particularly that 75 and 95% of the samples had melamine above the acceptable Maximum Residue Limit.  However, the estimated daily exposure to the two additives was below the allowed daily intake values, and the Hazard Index (HI) in the different milk samples ranged from 0.02 to 1.22, with one sample having HI>1.

    Conclusion

    The exposure risk of melamine and cyromazine among the general population is acceptable, and smaller HI values demonstrated no significant potential risk for the Nigerian population according to the recommended guidelines. Regulatory agencies are encouraged to step up their surveillance activities to forestall the inclusion of prohibited additives in local and imported milk to protect public health.

    Keywords: Dietary Exposure, Infant, Milk, Chromatography, High-Pressure Liquid, Nigeria
  • A. Shaalan, A. Al Khatib, S. Sakr, I. Sheet * Pages 47-58
    Background

    The olive oil in Lebanon play an essential role in the development of rural communities, providing a source of revenue and employment for most Lebanese population. Due to its high sensory quality and health benefits, olive oil is considered the most widely consumed oil in the Mediterranean area. Considering the factors that affect individual's knowledge of olive oil and consumer purchasing behavior can be difficult due to the diverse culture in Lebanon. The purpose of the current study was to evaluate the level of knowledge and the impact of sociological-demographic factors on the knowledge and consumer purchasing behavior towards olive oil among the general population in Lebanon.

    Methods

    A cross-sectional study was conducted in Lebanon between January and March 2021 in which 604 responses have been collected. The questionnaire made up of four dimensions: socio-demographic characteristics, knowledge, sources and usage of olive oil; and consumer preference part. IBM SPSS V22 was applied to analyze the data for descriptive statistics and chi-square test for independence. p-value of less than 0.05 was employed as the level of significance

    Results

    The findings revealed that the majority of the participants had only one or two uses of olive oil in their daily life and had minimal knowledge towards it. The association between the knowledge of participants about nutritional properties of olive oil among different sociological-demographic factors as governorate, gender, age and marital status was statistically significant (p<0.05). Participants with poor knowledge about olive oil were highest in Beirut (37.0%) compared to Akkar-North (3.1%). Furthermore, the consumers preferred southern, dark, and strong tasting olive oil in terms of region, color, and flavor with a statistically significant association (p<0.05).

    Conclusion

    Our findings highlighted the need to improve consumer awareness of the olive oil's benefits, which will influence their purchasing decisions.

    Keywords: Consumer Behavior, Knowledge, Lebanon, Olive Oil, Sociodemographic Factors
  • L. Mato, Z. Damani, J. Spahiu, E. Halimi, B. Seiti, D. Topi* Pages 59-68
    Background

    Today, mycotoxins are considered critical contaminants in foodstuffs produced by fungi, highlighting the importance of food safety to human health. The toxigenic fungi invasion and mycotoxin production are highly variable and depend on climate, plant, and agronomic practices. Among these, Aflatoxins (AFs) are considered the most potent toxins. This study investigated the fungi presence and AFB1 contamination in corn and wheat grown in Albania during the 2022 harvesting year.

    Methods

    Wheat samples were collected during the summer, while corn during the autumn, and further analyzed. Mycological contamination assessment applied the Verband Deutscher Landëirtschaftlicher Untersuchungs ̶ und Forschungsanstalten (VDLUFA) procedures. The AFB1 levels were measured using the Enzyme-Linked Immunosorbent Assay (ELISA) method. The MATLAB R2016b software was applied to perform statistical analysis. The Estimated Daily Intake on AFB1 was calculated to evaluate human exposure.

    Results

    The genera Alternaria, Aspergillus, Cladosporium, Fusarium, and Penicillium were isolated, with higher rates of contamination in corn and the highest frequency Penicillium genus (77.89%). The Korça region presented a higher fungal load, 104 Colony Forming Unit (CFU)/g in corn. The AFB1 incidence (88.23%) in corn, was significantly higher than in wheat (4.91%). Additionally, the maximum level in corn was found 69.120 μg/kg, while in wheat, only 0.402 μg/kg. None of the wheat samples, in contrast to the 41.18% of corn samples, exceeded the threshold when referring to the respective Maximum Residue Levels.

    Conclusions

    Our observation indicates a higher rate of AFB1 contamination in corn than in wheat. The high concentration levels and contamination incidence in corn require targeted interventions to reduce the AFB1 amounts. Strengthened regulation based on scientific evidence can reduce contamination outbreaks, economic implications, and potential benefits, such as increased consumer trust. Our study indicates that the exposure to AFB1 originates from corn consumption among the adult population.

    Keywords: Food Safety, Zea Mays, Triticum, Aflatoxin B1, Enzyme-Linked Immunosorbent Assay