digestive method

This study aims to determine the incidence of Sarcocystis sp. Infection in slaughtered Cattle in the abattoir of Khoy, Iran by digestive method. From March to September 2017, 204 slaughtered Cattle were investigated for the presence of Macroscopic Sarcocystis by direct observation. Each investigated cattle were classified into groups of age, sex and infected muscle tissue. All tissue samples were sectioned into 2-3 mm slices and carefully observed for probable macroscopic cysts. The sections were then examined using the peptic digestion method. Our study showed that the digestion method is useful to identify infected samples. According to the results, 11 out of the 204 cattles (5.39%) were diagnosed as being infested with macroscopic cysts. Microscopic sarcocystis infection in cattle slaughtered in the area was seen. About 94 cattle (46.08%) were diagnosed as positive infasted Sarcocystis species by the digestion method. The data analysis indicated that there is a statistically significant difference between age groups, and the infection rate increased with age (P<0.05). The infection rate was independent of sex, and the difference between males and females was not significant (P>0.05). Also, there was a significant difference between prevalence of Sarcocystis infection in different examined muscles(P<0.05) and most microcysts were diagnosed in skeletal (96.81%) and esophageal muscles (88.30%) in cattle and for buffaloes, microcysts were diagnosed in all infected skeletal muscles and esophageal muscles with 92.31%, was the second tissue with high prevalence.

Sarcocystis is composed of 130 species of heteroxenous coccidian and differ in terms of life cycle and pathogenicity. This parasite is common between humans and livestock and human being infected by eating raw or semi-ruminating meat. The infection is a global outbreak, causing many diseases in many animals and causing many health and economic losses. The aim of this study was to determine the rate of contamination of sarcosystis in slaughtered livestock in Semnan abattoir using compression and digestion methods. Hence during the winter of 1396 and spring of 1397, 334 cattle and 334 sheep and goats (evenly sheep and goats) were randomly selected at abattoir and examined by digestive and compression methods for the presence of Sarcocystis cyst. The mean of contamination of Semnan in cattle was determined using digestive method of 87% and with compressive method of 60%, in goats by digestive method 83.33% and using compressive method 59% and in sheep by digestive method 81% and with the compressive method was 59.52%. The results showed that the digestive method was the most sensitive method for detecting sarcocystis in abattoir in Semnan. Regarding the high prevalence of sarcosysts, the complete cooking of meat it should be considered, regardless of the apparent inspection outcome.

Sarcocystis is an obligatory intracellular protozoan parasite that can cause digestive disorders in infected patients. This study aimed to determine the incidence of Sarcocystis infection in slaughtered sheep of the Khoy abattoir, Iran. For this, 204 sheep carcasses were examined by visual observation and then further analyzed by digestion method. Based on the results, 5.88% of the studied carcasses were diagnosed with macrocytic observation. In the microscopic examination, 42.65% of the cases were found positive. Data analysis showed a statistically significant difference between contamination rate in different age groups. The occurrence rate increased with age (P < 0.05), while the intensity of infection was independent of the gender. There was also a statistically significant difference between the contamination rate among different muscles (p <0.05). In other words, the highest microcyst contamination observed in skeletal muscles (90.80%). Meanwhile, the lowest microcyst contamination was observed in the heart muscle (29.88%). This study showed the necessity of microscopic observation of the Sarcocystis contamination in the sheep carcasses.

Sarcocyst is a worldwide zoonosis that infects many humans and animals. From the point of view of health and economy, it has a large amount of loss for many countries. The parasite is one of the most common parasites in many humans, wild mammalians, birds, poikilothermic animals. Some of them are responsible for severe or fatal diseases in their intermediate hosts. In this study, the rate of infection to sarcocyst has been investigated in sheep by two methods macroscopic and digestive method, in the slaughterhouse of Borujerd, Iran. Sixty carcasses were randomly selected during two stages in: In the first stage, oesophagus, diaphragm, heart, scapular and femoral muscles have been inspected that in total 17 (28/33%) from of them were infected by the cyst. In the second stage, these parts were tested by digestive method (Pepsin: 2.5 grams; Chloric acid: 10 cc; Phosphatebuffered saline 100cc) and then the smears were stained by Giemsa. The specimen that had bradyzoite considered as the positive. In conclusion, 56(93/33%) of samples had bradyzoite. the study showed a significant differences between two methods (P<0.01). Microscopic method was more sensitive than the other method.