Analysis of endothelial nitric oxide synthase (eNOS) gene polymorphism Glu 298 Asp as a risk factor of coronary artery disease in a sample of Iranian population

Genetic factors contribute to the pathogenesis of Coronary artery disease (CAD). Among many polymorphisms of the eNOS gene, the Glu298Asp variant has implications in coronary artery disease (CAD). The aim of the present study was to investigate the association between CAD risk and Glu298Asp polymorphism of the eNOS gene. In this case- control study, the eNOS gene polymorphism was analysed in 92 Iranian patients with coronary artery disease and 93control subjects. The Glu298Asp polymorphism of the endothelial nitric oxide synthase gene was determined by polymerase chain reaction and restriction fragment length polymorphism. There were significant differences between the control and patient groups for the eNOS3 genotypes. In addition, the family history of coronary artery disease, hypertension, dyslipidemia and eNOS TT genotype were independent risk factors of coronary artery disease. Those with TT genotypes hd 3.5 times (odds ratio = 3.57) risk of coronary artery disease compared with the control group. These results suggest that coronary artery disease is associated with the Glu298Asp polymorphism of the endothelial nitric oxide synthase gene in our population.