Comparison of Methods for Detection of Listeria Monocytogenes in Food Samples Contaminated

Aims and Listeria monocytogenes bacteremia in pregnant women with various complications in the fetus, including Be aborted. However, Listeriosis can detect and isolate the bacteria to be cultured,due to being time consuming, low efficiency due to low number of bacteria and other bacteria problem in the differential diagnosis in many laboratories using this method typically does not. Also becomes less important as the methods of immunology, molecular detection of bacteria in samples from the patient or suspected food has high specificity. In this study the molecular method in comparison with other methods used to differentiate bacteria. In this study, 152 cases of suspected laboratory routinely referred to Ma ssoud laboratory, who had consumed contaminated food samples were collected, and of antibodies to Listeria monocytogenes Were studied by IFA Method. The deadly samples using molecular methods of DNA extraction and PCR method were evaluated that all of the standard molecular techniques were used. 47% of all samples (71) in a dilution of 1/100 and 1/400 was positive in the immunology method. The bacterial cultures were positive in 41 cases, but the molecular method of total samples of suspected cases, 53 were positive. Due to the rise of virulent Listeria infection and also in connection with abortion, the importance of contamination by bacteria important in the foods and recently most doctors recommend screening pregnant women in terms of Listeria contamination. According to WHO estimates are not reliable methods antiserum and molecular methods and culture is very important, especially in screening pregnant women is high specify molecular methods.