Mycorrhizal symbiosis of Pyrus glabra and comparison of colonization percentage and spore abundance in spring and autumn

Mycorrhizal symbiosis facilitates establishment of plant by increasing water and soil elements absorption such as N, P and K and rhizosphere distribution. It also increases soil biodiversity and microbial population as well as plant resistance against pathogens and stress. In this study thirty individuals of natural Pyrus glabra in Bazoft were selected. To find out the interactions between arbuscular mycorrhizal and soil elements, root samples were obtained during spring and autumn. Arbuscular mycorrhizal fungi spores were isolated by wet sieve and centrifugation by means of sucrose. Then roots were stained and colonization percentage was measured. Seven species of Glomus and 2 species of Acaulospora were characterized. Significant difference was found between Arbuscular mycorrhizal colonization percentage and spore frequency in spring and autumn. Significant correlation was observed between colonization percentage and total nitrogen in spring.