Study of In-situ Glutathion Peroxidase Gene Expression in Growth stages of Brassica napus at Drought Stress Condition

In order to transiant Glutathion Peroxidase gene expression, protein content, chlorophyll and oxidative level in Brassica napus (Falcon cv.) in response to drought stress condition, the experiment has been carried out in RCBD format design with four replicates under research greenhouse condition. The drought stress treatments were included -0.5, -1, -2, -4 and -8 bar that applied in each plot by using of equivalent water. Field capacity (FC) has been used as control. The periodic water treatment has been applied every week at the four leaves growth stage. Random leaf samples have been collected to measure all mentioned trails at the growth stages (Stem elongation, flowering, Pod stage, Physiologic maturity). Root samples have been collected for gene expression at all mentioned growth stages. For grain analysis samples have been collected of maturity to measure oil percentage, protein and gene expression. The result showed leaf protein declined sharply by plant age and drought intensity, beside grain protein content showed more stability in compare with leaf protein at drought condition levels. Net grain oil percentage (grain yield * oil percentage) showed more fluctuation in compare with grain protein at drought treatment levels. Although this fluctuation was rather less for -0/5 and -1 bar in compare with other drought treatments. Leaf chlorophyll content decreased by increase drought treatments. This decrease was low up to -1 bar drought treatment and rather increase after the level of -1 bar. In contrast, the cellular oxidative level (TBARM) increased by intensity drought level, this has been caused by AOS (Active Oxygen Species) levels. The activity of glutathione peroxidase gene showed sigmoid trend mostly in all plant organs (leaf, root, pot, grain). Basically, this has emplied on importance and gross range of activity this gene.