A Survey Frequency of TEM-1 Gene In E. coli Strains Isolated from Clinical specimens in Damqan City

In recent years, prevalence of Extended Spectrum β- Lactamase (ESBLs) Producing bacteria were increased. These bacteria can hydrolyze the most of β-lactam antibiotics and are known as one of the main problems in Drug resistances and bacterial infection control programs and its illness treatment. TEM-1 β-Lactamase gene product is one of the most important ESBLs β-Lactamase in Enterobactriacea, such as E. coli, Which is the most important factor in E. coli resistant strains to β-lactam drugs and cause Nosocomial infections. In this study, The frequency of TEM-1 gene in Damghan Clinical isolated of Extended Spectrum β-Lactamase (ESBLs) Producing E. coli was surveyed by PCR method. In this project, information of patients were collected through special forms and then, transfer 70 bacterial isolated to laboratory, The specimens were tested and bacterial type were Identified, examined for β-Lactamase producing by combined disk method (according to CLSI) and then Extended Spectrum β-Lactamase Producing bacteria were surveyed by using PCR method to determine the TEM-1 gene frequency. From 70 E. coli isolate, 27 specimens were β-Lactamase Producer. 10 of 27 specimens (%37/04), had TEM-1 β-Lactamase resistance gene. These results indicated the importance of TEM-1 gene in β-lactam antibiotics hydrolysis between E. coli strains isolated in Damghan. The identification of this gene frequency in clinical isolates such as E. coli strains can speed up the process of diagnosis and treating of patients.