Comparison of allergic reactions of Trichophyton rubrum and Microsporum canis in guinea pig and planning of Tri r4 gen specific primer

Trichophyton rubrum is a wild spread fungi which cause the Dermatophytosis and approximately 80-93% of chronic or recurrent/recurdescent dermatophyte infections are created by trichophyton rubrum. Tricophyton proteins are members of different enzymatic families and dual role of these proteins in fungal pathogenesis as well as their allergenic behavior. Among trichophyton rubrum allergens, Tri r 2 and Tri r 4 can be mentioned. The present research aims to investigate the allergenic behavior of trichophyton rubrum and microsporum canis. Initially, the fungi trichophyton rubrum was cultured on PDA medium. Their conidia were subsequently gathered by sterilized PBS. DNA was extracted and PCR experiment was fulfilled using the specific primers Tri r4. Additionally, Guinea pig was used to study the allergenic behavior of the fungi trichophyton rubrum and microsporum canis. To confirm that the fungi were allergenic, total IgE antibody was assayed by ELISA method. Also, in CBC test performed on Guinea pig blood samples, percentage of blood cells especially eosinophils were evaluated. Conidia were confirmed by using PCR test for Tri r4. Results of allergenic behavior indicated that more IgE antibody, also neutrophil and eosinophil were produced in the Guinea pig in which trichophyton rubrum was inoculated and has a significant different compared to control group, whereas no significant different was observed in comparison with control group in the Guinea pig in which microsporum canis was inoculated. The present research indicates direct effects of allergens in enhancing titer of IgE antibody.