Monospecific Polyclonal Antiserum Production against Bacterial HBsu

HBsu protein in Bacillus subtilis is a member of histone like protein family HU. The protein has an important role in structure and function of bacterial nucleoid. In sights of its importance and similarity between the members of HU family, in this study the purified expressed HBsu, as an immunogen, was injected in rabbit until the produced polyclonal antiserum was used in the next research about identification of the other HU family members. The recently expressed HBsu protein from hbs gene of Bacillus subtilis ATCC6633 was injected in two male rabbits according to a twenty-day schedule and immunoblotting was done for antiserum production checking. After twenty days, the antiserum against HBsu was produced and the presence of the antiserum was confirmed by immunoblotting. Also, extracted histone like protein from Halobacillus karajenesis was used for checking of its ability in identification of HBsu like protein that the answer was positive. The used method was rapid and with high ability for antiserum production against HBsu protein. The similarity between Halobacillus karajenesis HU and Bacillus subtilis HBsu is enough for the identification of this HU by antiserum against HBsu.