The study of protective effects of Saccharomayces boulardii probiotic on Salmonella typhimurium intestine lesions in rat

Salmonellosis is an infectious disease of all animals species caused by a number of different species of Salmonellae and manifested clinically by preacute septicemia or enteritis. Much recent investigation suggested that some probiotics such as Saccharomayces boulardii can prevent pathological changes caused by Salmonellae in vivo. This study was conducted to investigate the protective effects of Saccharomyces boulardii on intestine pathologic lesions of Salmonella typhimurium in rat. Thirty Conventional male rats were divided in to three group (A,b,C).Rats in group B and C received 1 ml of saline contained 107 ¬and 108 CFU of Saccharomyces boulardii orally for 5 days. Respectively animals in group A (as control) received only 1 ml saline as experiment groups on day five all animals were challenged orally with 107 Salmonella typhimurium on day 10, firstly all rats challenged with bacterium then all animals were euthanised. specimens of intestine were fixed in 10% buffered formalin solution for histological examinations and translated to pathologic library. The result of intestine histopathologic examinations was investigated by fisher exact test. The result indicate that all control groups show diarrhea and infilteration of inflammatory cells in mocosa compared with rat in experiment group that didn’t show any marked pathological changes in the intestine. This study indicates that sacharomyces boulardii hase protective effect on Salmonella typhimurium infection in rat and histological examination confirm this results.