Application of Ammoniphillus as Probiotic in Spinach Cane for Oxalate Reduction

Up to 75% of all urinary stones are composed mainly of calcium oxalate. Spinach species have been identified as high-oxalate containing plants. The aim of this study was the isolation of oxalate degrading bacterium and evaluation of its application in reducing the oxalate content in spinach. The strain used in this experimental study was confirmed by molecular methods. Spinach was purchased from the market، and the ex-crude was obtained. Bacterial biomass was prepared and the wet weight was determined. The diluted and non-diluted spinach ex-crude was mixed with bacterial biomass and then incubated. Also، diluted spinach extract was mixed with enzyme suspension of strain and then incubated. Ultimately، oxalate concentration was determined with oxalate diagnostic kit. The data was analyzed using the Microsoft Excel program version 2007. The strain (Ammoniphilus oxalaticus DIM) was submitted to NCBI website with access number HQ398365. This strain could decrease 98. 3% and 51. 54% oxalate content in diluted and non- diluted spinach ex-crude respectively. Ammoniphilus oxalaticus DIM strain is a good candidate for probiotic activities in the body. Moreover، it could be used in canned products، such as spinach، to reduce the amount of their high oxalate content.