Detection of Neisseria meningitidis in Pediatrics Meningitis by PCR, in Comparison to Culture and Agglutination Tests (Short Report)

Bacterial meningitis especially meningococcal meningitis is a serious and sometimes fatal infection. The aim of this study was the determination of meningitis disease by routine and molecular assay s. CSF samples were collected from 275 children and agglutination، bacteriological tests were achieved. DNA extraction was done according to phenol-chlorophorm method and PCR was carried out by the specific primers. The mean age of the patients was 42±2 month. Younges was 11 days and the oldest child was 11 years old. Meningococcal meningitis was more common in the male gender. Totally، Neisseria meningitidis was found in 3 (1. 1%)، 5 (1. 8%) and 10 (3. 6%) by culture، agglutination and PCR assays، respectively. One of the most common etiological agents of meningitis is Neisseria meningitidis in the Azerbaijan state. Molecular diagnosis has higher sensitivity and specifity in comparison to culture and agglutination tests.