Molecular Detection of Chlamydophila Psittaci among Dead and Suspected Cases of Parrots in Tehran

Chlamydophila is an organism similar to geram negative and interacellular bacteria that is classified in Chlamydiacae family. Various types of Chlamydophila exist in this family that can infect their hosts. The species that infects birds is Chlamydophila psittaci. It can cause diseases in humans. The disease is transmitted via touching or secretions and the infected objects of infected birds, especially psittacine birds. The purpose of this research is the Molecular detection of Chlamydophila psittaci among dead and suspected cases of psittacine birds in Tehran. In this study, 23 excrement swabs of suspicious parrots and 7 cloacal swabs of dead but suspicious to the existence of Chlamydophila were investigated. The samples were kept in -20 C degrees up to the extraction time. The method of DNA extraction was performed by Celica filters, and then was examined by using Nested PCR Method based on 16SrRNA genom of chlamydophila. The findings obtained from 10 positive cases among 30 tested samples. The results showed the importance of giving more information to the psittacine birds’ keepers about Chlamydophila in order to have healthy parrots and to prevent their keepers from the disease.