Evaluation of Seed Germination, Growth and Plant Anatomy of Salsola arbuscula Pall. in Salt Stress in In Vitro

In this study, the effects of NaCl on seed germination leaf and stem anatomy and growth parameters were investigated in 10 and 60 days-old of Salsola arbuscula species. The species seeds were cultivated in WA (0, 100, 200, 250, 300 mM NaCl), and MS (0, 100, 200, 250, 300, 350 mM NaCl) in vitro media. Then 10 and 60 days-old plants were used for studying seed germination and growth parameters. In addition to leaf anatomy of 60 days-old plants in MS (0, 100, 200, 250, 300, 350 mM NaCl) and after recovery in MS (300, 350, 400 mM NaCl), germination percentages were also investigated. Results showed decline in germination percentage and ability of recovery in germination percentage. Also growth and anatomical parameters showed wide varieties in 10 and 60 days-old plants with increasing NaCl concentration. S. arbuscula is a halophytic plant that shows a better growth in low concentration of NaCl than control. Also the species protects its water content along the stress condition on the contrary glocophytes and has germinating ability. These characters help to survival of the species.