An analysis of the antioxidant compounds in the alcoholic extractions and the juice of elm-leaf blackberry or Rubus anatolicus (Focke, i.e. Focke ex Hausskn.) during the ripening period

Elm-leaf blackberry or Rubus anatolicus (Focke, i.e. Focke ex Hausskn.) belongs to the Rosaceae family and Rosoiedeae subfamily. It is a wild plant which grows in abundance in Golestan region. It is used as a strong anti-inflammatory and antiseptic substance in conventional medicine. In this study, un/ripe elm-leaf blackberries were randomly gathered from two different regions of Golestan (i.e. Hasan Abad village of Jelin and Livan-e-gharbi village in Bandargaz). The total amounts of polyphenol and flavonoid in the methanol and ethanol extractions and the bramble juice were respectively determined using the folin-ciocalteu reagent and the aluminum chloride colorimetric procedure by means of a Spectrophotometer. The amounts of the two phenol compounds were higher in the ripe brambles in comparison to the unripe ones. Considering the regional differences, there was only a significant difference among the unripe brambles regarding the methanol extraction in the phenol content. However, there was a significant difference (P<0.05) between the two types of brambles, regarding the flavonoids obtained from both of the methanol extractions and the juice. Respectively, the total amounts of phenol and flavonoid in the methanol and ethanol extracts were generally higher than the other two extractions.