Effect of operational variables on aqueous enzymatic oil extraction from soybean

In comparison with traditional extraction methods، aqueous enzymatic extraction of oil from oilseeds is a recent clean technology. This paper reports work performed at laboratory scale to extract soybean oil by aqueous enzymatic extraction method. In the present work the influence of enzymes concentration، extraction time، dilution ratio، particle size and 3 interactions in the final yield are evaluated and process parameters have been optimized by Taguchi method. 16 extraction experiments carried out، statistical analysis showed that particle size was the most significant variable in oil extraction. Themaximal oil extraction yield was 61. 42%.