Phylogenetic analysis of Neuraminidase gene of avian influenza H5N1 subtype detected in Iran in 1390(2011)

Among the various subtypes of avian influenza viruses، an H5N1 subtype virus with high pathogenicity is of great importance. The aim of this study was to determine the Phylogenetic analysis of neuraminidase gene of avian influenza virus subtype of the H5N1 in Iran in 1390. In this experimental study، two swab samples from chickens with suspected symptoms of avian influenza were tested by the World Health Organization recommendation. The neuraminidase gene of positive samples was amplified by RT-PCR technique. After sequencing the phylogenetic studies were analyzed using MEGA5 and Megalign. Phylogenetic analysis showed that the virus belongs to the Clade 2. 3. 2. 1 which is highly similar to the viruses that are identified in Mongolia in 2010. Also in the stem of this virus neuraminidase protein a number of 20 amino acid has been deleted at position 69-49. Due to findings of this study، it seems that the virus has entered by migratory wild birds with the origin of Mongolia.