Effect of Amylase, Papaein and Pepsin enzyme solutions on Candida biofilm formed on acrylic resin plates

Denture stomatitis results from colonization of oral Candida on the surface of denture acrylic base. To control this infection،Candida biofilm formation must be prevented using mechanical and chemical decontamination. The purpose of the present study was to evaluate the effect of Amylase، Papaein and Pepsin solutions on removal of Candida Albicans plaques formed on acrylic resin plates. In this experimental study Candida biofilm was formed on 220 acrylic resin plaques. The average attached Candida cells on 20 randomly selected plates were determined by staining of the plates with Crystal violet and observing them under an optical microscope. 200 contaminated plates were then randomly divided into 4 groups of 50، immersed in 100µg/ml Amylase، Pepsin، and Papain enzyme solutions as test groups، as well as sterile distilled water as negative control group. They were kept in solutions for 8 hours in room temperature. The mean attached Candida cells were determined and data was analyzed using ANOVA and T- test using SPSS software. The average Candida cells attached on to acrylic resin plates on each visual field after decontamintion with Papain solutions was 2. 39±0. 76، Pepsin 7. 14±1. 47 followed by Amylase 8. 22±1. 29، but it was 9. 66±1. 54 in negative control group. The results showed significant differences (P=0. 001). Papain enzyme cleaned 78. 9% of attached Candida cells on acrylic resin plates، whereas Amylase showed minimum antimicrobial properties by removing only 27. 4% of attached Candida cells. Papain solution has maximum effect on removal of Candida from acrylic resin plates