Screening of new polymorphisms of subunit 1 of cytochrome oxidase gene in COPD patients
Author(s):
Abstract:
Aim and
Background
Chronic Obstructive Pulmonary Disease (COPD) is caused by inflammation and destruction of a part of lung and usually appears after age 50. Considering the muscle weakness، energy limitation and problem with oxygen consumption in COPD patients، we analyzed the mitochondrial cytochrome c oxidase (COX) gene to find the relationship between COXI and COPD disease. We studied 65 patients (71±8 years old) who were clinically stable and 50 healthy people (56±4 years old) as a control group. DNA was extracted and prepared according to the standards. Material And Method
Isolated DNA was used for amplification of COXI gene. In this study، screening four new polymorphisms using ARMS Real time PCR was performed in COXI. Results
PCR conditions were optimized empirically for screening 4 polymorphisms by ARMS Real time PCR. Significant difference polymorphisms between COPD patient and normal control was foundConclusion
The light note of polymorphism was found at 6060 locus، which is the only polymorphism that leads to an amino acid change. Increasing the value of this polymorphism in patients with COPD than normal individuals can make this polymorphism a risk factor in COPDKeywords:
Language:
Persian
Published:
New Cellular & Molecular Biotechnology Journal, Volume:3 Issue: 11, 2013
Pages:
29 to 35
magiran.com/p1171390
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یکساله به مبلغ 1,390,000ريال میتوانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
توجه!
- حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران میشود.
- پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانههای چاپی و دیجیتال را به کاربر نمیدهد.
In order to view content subscription is required
Personal subscription
Subscribe magiran.com for 70 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!