Investigating the frequency of MSPI polymorphism of APOA1 gene in type II diabetic patients and comparing it with this frequency in nodiabetics

Diabetes is a multifactorial disorder and many genes related to diabetes are detected. APOA1 is located on chromosome 11 and has role in the lipids metabolism. There is close relationship between lipids and carbohydrate metabolism. Therefore it is a possibility of relationship between APOA1 gene and carbohydrate metabolism. In this case control study the incidence of APOA1 polymorphism in patients with type 2 diabetes were investigated. In total 200 diabetic patients referred to Yazd Diabetes Research Center were investigated. Blood sample of these patients and 200 individuals without diabetes as controls were collected. DNA was extracted and then PCR-RFLP method was used. The genotype M1+/+ was detected in 57. 6% of cases and 58. 7% of controls، while genotype M1+/- was detected in 36. 7% of cases and 34. 2% of controls and genotype M1-/- in 5. 6% of cases and 7. 1% of controls. Regarding M2 genotype، M2+/- and M2-/- were reported together and the results indicated that the rate of M2+/+ was 93. 8% in patients and 94% in controls while the rate of the other two genotype were 6. 2% in patients and 6% in controls. Our study showed that there is no significant difference in the rate of APOA1 gene polymorphism between diabetic patients and control group. This indicates that there is no relationship between APOA1 polymorphism and diabetes. Therefore it seems that the major role of APOA1 is in lipid metabolism and not carbohydrate metabolism.