Evaluating the Effect of IL-27-Transfected Mesenchymal Stem Cells on Certain Histological and Immunological Parameters in a Mouse Model of Experimental Autoimmune Encephalomyelitis
Author(s):
Abstract:
Background
Mesenchymal stem cells (MSCs) by having immunomodulatory and neuroprotectivity properties could be a suitable candidate for the treatment of inflammatory and neurodegenerative diseases. Furthermore، by genetical manipulation، it is possible to potentiate the therapeutic characteristics of these cells. The aim of this study was to investigate the effect of IL-27-transfected MSCs in a mouse model of experimental autoimmune encephalomyelitis (EAE). Methods
MSCs were isolated from the bone marrow of C57BL/6 mice and were trnasfected with mouse IL-27 gene by using a lentiviral vector system. On days 15 and 22 post EAE induction، one million of these cells were injected to EAE-affected mice. Finally، all the mice were sacrificed; and histological and immunological assays were performed to investigate the myelin status and infiltration rate of immune cells into the central nervous system (CNS) tissues and measure certain cytokines، respectively. Findings
IL-27-transfected MSCs could ameliorate disease in the EAE-affected mice. Besides، they could diminish the infiltration rate of immune cells into the CNS، and inhibited the myelin destruction. Moreover، IL-27- transfected MSCs could enhance IL-4 and IL-10، and reduce IL-17 production from the EAE mice splenocytes. Conclusion
IL-27-transfected MSCs could be considered as a therapeutic tool for the treatment of inflammatory diseases including multiple sclerosis (MS). Changing dose and rout of administration، however، would be suggested to achieve better results.Keywords:
Language:
Persian
Published:
Journal Of Isfahan Medical School, Volume:31 Issue: 249, 2013
Pages:
1270 to 1284
magiran.com/p1190304
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یکساله به مبلغ 1,390,000ريال میتوانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
توجه!
- حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران میشود.
- پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانههای چاپی و دیجیتال را به کاربر نمیدهد.
In order to view content subscription is required
Personal subscription
Subscribe magiran.com for 70 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!