The association between glutathione s-transfrases T1, M1, and P1 polymorphisms with end stage renal disease

End stage renal disease or ESRD is a progressive and irreversible deterioration in renal function in which the body loses ability to maintain metabolical balance، fluid and electrolytes. Glutathione s-transfrases (GSTs) are members of multigenic family which have essential role as an antioxidant in the cells. In this study، the polymorphism of GST، T1، M1 & P in ESRD patients were compared with those of control groups to determine the possible relationship between polymorphism of these enzymes and ESRD.

136 of ESRD patients as experimental and 137 of healthy individuals as control group were examined. The GST T1 & M1 genotypes were determined in all individuals with multiplex- PCR and GST P1 polymorphism was determined with PCR-RFLP.

The distribution of GSTT1& M1 of T1+-M1+ (wild)، T1+-M1¯، T1¯-M1+and T1¯-M1¯ (null) genotypes in control group were 45 (32. 8%)، 40 (29. 2%)، 28 (20. 4%) and 24 (17. 5%) and in experimental group were17 (12. 8%)، 46 (34. 6%)، 21 (15. 8%) and 49 (36. 8%) respectively (P<0. 001).

As the reduction of GST T1+-M1+ (wild) was observed with more frequency in patients with ESRD compared to control group (12. 8%vs. 32. 8%)، this genotype can be associated with the risk of ESRD.