Comparison of KIR/HLA genotypic analysis in the Lur and Iranian populatins

Killer-cell Immunoglobulin-like Receptors (KIR) are the members of inhibitory and activating receptors expressed chiefly by the natural killer cells (NK). KIR proteins operate as receptors detecting human leukocyte antigen class Ι molecules (HLA). KIRs and their class Ι HLA ligands contribute in the pathogenesis of many kinds of diseases. The aim of this study was to genotypic analysis of KIR/HLA in the Lur population for the first time.

In this study، 100 unrelated healthy Lur individuals were KIR and HLA ligand typed by polymerase chain reaction-sequence specific primers genotyping assay. Finally، the frequency of KIR and HLA ligand genes and genotypes، as well as KIR/HLA combinations in the Lur population was compared with the Iranian population.

Twenty two KIR genotypes and all of the KIR genes were observed in the Lur population. The most prevalent non-framework genes were KIR2DL1 and KIR2DP1 with the frequency of 98% and KIR3DL1 and KIR2DS4 with the frequency of 96% in the Lur population. AA genotype with the frequency of 29% was the most frequent KIR genotype ever regarded in the Lur population. The number one genotype with the frequency of 35% was the most frequent genotype in the Lur population among HLA ligand genotypes. The most common inhibitory and activating KIR/HLA combinations were KIR2DL2/3+HLA-C1 with the frequency of 75% and KIR2DS2+HLA-C1 with the frequency of 47%، respectively. Discussion and

The results illustrate that the frequency of KIR genes and genotypes، HLA ligands and the KIR/HLA combinations has total similar features with the Iranian population، but it is still unique by increasing or decreasing of some frequencies in the Lur population.