Aerial Parts of Scrophularia Striata Bioss on Escherichia Coli, Staphylococcus Aureus and Bacillus Cereus

Side effects of chemical drugs led to a growing interest to use natural antibacterial compounds like extracts of herbs. The aim of this study, evaluation of phytochemical constituents and the in vitro antimicrobial activity of the root and aerial parts of Scrophularia striata against the bacteria of Escherichia coli, Staphylococcus aureus and Bacillus cereus. In this study, anti-microbial effects of sequential extracts from root and methanolic extract of aerial part, minimal inhibitory concentrations of bacteria and minimal bactericidal concentrations of bacteria were determined against the bacteria of Escherichia coli, Staphylococcus aureus and Bacillus cereus by using the disk diffusion and dilution method. DMSO was used as a negative control whereas nalidixic acid and ampicillin were used as a positive control. Collected data were analyzed using one-way ANOVA test. The results showed that the root and aerial parts of Scrophularia striata contain flavonoids, glycosides, saponins, tannins and terpenoids. The ethylacetate and chloroform extracts of root showed no inhibition zone of the bacterial growth. Whereas, the methanolic extract of root was the most effective one against Bacillus cereus with the inhibition zone of the bacterial growth 21 mm, MIC 60 mg/ml and MBC 70 mg/ml. The methanolic extract of aerial part has the least effect on Escherichia coli. The present study showed that the methanolic extract of root and aerial parts of Scrophularia striata has antibacterial effect especially against Bacillus cereus. Therefore, more studies are needed for identification of various compounds and attainment of new formulation of these components in this plant. Key words: