Measuring Zinc, Copper, and Calcium in wheat spike seeds during the growth period

The relative concentration of zinc, copper, and calcium in the seed of different parts of spike were evaluated in an attempt to select the best seeds in wheat spike (Triticum aestivum L. var. PBW-343). The spikelets in the main spike were divided into proximal, middle, and distal regions. The grains in each spikelet were divided into basal and apical parts. The concentrations of zinc, copper, and calcium were at the lowest level in the 14th day. Seeds in the basal part of the middle region spikelet were in a higher level in comparison to the grains of the other parts. The basal seeds in the distal spikelet had a higher concentration of calcium compared to the other parts.