Effect of Embryonic Cerebrospinal Fluid on Proliferation and Self-Renewal of Wistar Rat Neuroprogenitor Cells

During recent years many studies have suggested some developmental roles for embryonic cerebrospinal fluid. Here we examined the effect of embryonic cerebrospinal fluid on proliferation and self-renewal of embryonic ventricular zone derived neurosphere. Cortex from 15. 5 day old embryonic Wistar rat dissected out and enzymaticaly dissociated to form single cell suspension. Cell suspension seeded in DMEM/F12 medium supplemented with N2 and mitogenes (10ng. ml-1 EGF and 20ng. ml-1 bFGF). The CSF-treated culture from different embryonic ages (E16، E18، and E20) in 10/100 ratio (v/v) was considered as experimental groups. Neurospheres number was counted for proliferation assay and cell viability evaluated with MTT assay. The data were analyzed with One-way ANOVA with turkey’s post hoc test. Embryonic cerebrospinal fluid (e-CSF) enhanced neurosphere number. Also، embryonic cerebrospinal fluid (e-CSF) enhanced cell viability and growth. Embryonic cerebrospinal fluid (e-CSF) enhanced proliferation and viability of neural progenitor cells in age dependent manner. Moreover، this fluid play important role in establishment of neuroprogenitor cells self-renewal.