Preparation of nanoniosomal Paclitaxel formulation and survey of it's Cytotoxic Effect on breast cancer cell line (MCF-7)

Aim and Paclitaxel is one of the drugs used in breast cancer. Use it as a side effect to weakening the bone marrow and peripheral neuropathy causes. Recently been shown that nanotechnology can be used to further reduce side effects، increase the efficiency of treatment. One of the nano-carriers for drug delivery، are niosomes. In this study nano paclitaxel niosomal with the ether injection methods and reverse-phase evaporation were prepared and studied. Aim of this study use of nanoparticles niosomal containing paclitaxel and cytotoxic effects on breast cancer cell lines. In this study، in order to improve the therapeutic effects and reduce side effects paclitaxel، drug with both methods by ether injection and reversed phase evaporation، was preparate nano-niosomal pegilation. Span 20، cholesterol، polyethylene glycol l2000 Dalton، paclitaxel، solution of MTT (0. 5 mg / ml) was purchased from Sigma. Ethanol and isopropanol and diethyl-ether from Merck Co. and culture RPMI 16-40 purchased from Invitrogen Co. and MCF-7cells were obtained from the Pasteur Institute Cell Bank of Iran. After a description of resulting nanoparticles indicated the manufacturing method، combined with polyethylene glycol exert a positive effect on the properties of nanoparticles. We Could encapsulation amount of paclitaxel in niosomal nanoparticles to be dramatically raise. In this study were identified by using nanotechnology and niosomal nanoparticle can be appropriate formulation of the paclitaxel «drug» prepared، Be more effective than the free drug the dosage for treatment is reduced and this reduces the side effects are On the other side، is a low-cost treatment.