Preparation of Electrospun Nanofibrous PLGA / Gelatin for Tissue Engineering

The tissue engineering with possible opportunity for repairing، replacing، maintaining، or improving the function of damaged tissue or organ had made a promising approach for treating human life quality. Nano-fibrous electrospun substrates which provide three dimensional substrate can mimic the extra cellular matrix to support the growth of cells. Combination of natural and synthetic polymers improves the strong signaling pathways and also proper cell behavior on the scaffold. In this study nano fibers were prepared with gelatin، PLGA and Gelatin/PLGA via electrospinning; and the cell growth and proliferation of fibroblast L929 on these scaffolds were evaluated. Gelatin was used to improve the biological property of the PLGA nanofibers. After making the Nano-fibers the SEM was used to take some to take some photos، then growth of fibroblast cells was analyzed using the SEM and reverse microscopics. Microscopic images showed that the first 24 hours of cell culture، cells behaved similarly in the three scaffolds but After 72 hours; the Gt/PLGA nanofibers provided a better substrate for adhesion and proliferation. While gelatin Nano-fibers had less growth rate after 72 hours. Also these results were confirmed by SEM. PLGA/Gt nanofiber due to activation of signaling pathways relevant in adhesion protein expression and replication are suitable for tissue engineering. This behavior may be very similar to the natural structure of the extracellular matrix.