The Survey on -592 Polymorphism of Interlukin-10 in Hepatitis B Virus Infected Patients

Infection with hepatitis B virus (HBV) may result in a number of different clinical outcomes. There are strong evidences in HBV infection that host genetic factors play a major role in determining the outcome of infection. Polymorphisms in the promoter region of interleukin 10 (IL-10) affect its production and confer susceptibility to inflammatory diseases. The aim of present study was to investigate the association between HBV infection and -592 polymorphism in the promoter region of the IL-10 gene in Iranian population. 100 HBV infected patients and 100 healthy individuals were randomly selected. Genomic DNA was extracted from blood buffy coat using the salting-out method. The IL-10-592 (A/C) genotypes were determined using allele-specific polymerase chain reaction (PCR) method. The PCR products were electrophoresed on 1. 5% agarose gel. The data were analyzed using chi-square test. The frequency of IL-10-592 C/C genotype was 16% in patients infected with HBV and 12% in healthy individuals and was not significantly different (P = 0. 5). Also، the frequency of C variant allele was 55. 5% in HBV infected patients and 53% in healthy individuals that was not statistically different (P = 0. 6). Based on our findings، there was not any relationship between IL-10-592 (A/C) polymorphism and susceptibility to HBV infection in our study population.