Effect of hydroalcoholic extract of pumpkin seeds on start of oogenesis and change of hormone-pituitary-ovarian axis in immature rats

Review: Effect of hydroalcoholic extract of pumpkin seeds’ assessment on start of oogenesis and Change of hormone-pituitary-ovarian axis in immature rats. Pumpkin Seed have been used in traditional medicine as a drug for improving sextual function and vigor. We did not find sufficient scientific evidence to support it. The present study was designed to evaluate of effect of hydroalcoholic extract of pumpkin seeds’ assessment on hormone-pituitary-ovarian axis and oogenesis in immature rats. In this experimental study، we used 30 immature female Wistar rats (weight range 100 to 80 g and 35-30 day olds) were randomly selected and divided them into five groups of six; three experimental groups، control and sham groups. Extract the experimental groups (200،100،50 mg/ kg dose of pumpkin seed extract) was injected intraperitoneally for 21 consecutive days. One day after the last injection، blood was taken from rats to investigate the sex hormones and their ovaries were removed for histological studies. The results showed significantly increased the mean serum FSH، estrogen and progesterone in experimental groups compared to the sham and control (P <0. 05). Secondary follicles in the experimental group compared to the sham and control groups showed a significant increase (P<0. 05). Body weight and weight of the right and left ovaries in the experimental group increased (P <0. 05). Amongst the other parameters، no significant differences were observed in all groups. Injection of hydroalcoholic extract of pumpkin seeds could significantly be effective on oogenesis and concentration of FSH، estrogen and progesterone hormones