Salmonella contamination of eggs of native Kohgiluyeh va Boyerahmad using PCR1 techniques and the evaluation of drug resistance
Abstract:
Background and Aim
Foodborne disease is a major health and economic problem in industrialized and non-industrialized countries. The purpose of this study was to investigate salmonella contamination of native eggs in kohgiloyeh & Boyerahmad province by PCR and evaluation of drug resistance. Methods
This cross-sectional study-descriptive study has done on 210 eggs which collected from native Kohgiluyeh & Boyerahmad.province. Biochemical tests were used to identification of b isolates acteria. Suspected salmonella reactions were tested by PCR with primers invA gene. The data were analyzed by chi-square test and Fisher. Results
The results showed that 14 eggs (6.66%) of all were contaminated with salmonella genus. The highest contamination area was seen in Dehdasht whereas the lowest area was shown in Charvsa, Dyshmvk, Lendeh and Basht. No significant correlation was found between the type and extent of contamination and the region. The most antibiotic resistance was seen to penicillin (100%). Conclusion
Microbial agents such as salmonella can cause food spoilage and disease. To avoid of resistance to salmonells species discriminate use of antibiotics in livestock and poultry should be recommended.Keywords:
Salmonella , Egg , drug resistance , invA , PCR
Language:
Persian
Published:
Armaghane-danesh, Volume:19 Issue: 2, 2014
Page:
128
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