GSK3β phosphorylation with DHEA in neural progenitor cells derived from Balb/c mouse embryos brain

Studies have shown that any disruption in wnt signaling pathway is associated with Alzheimer disease (AD). One of the important molecules involved in activation or inactivation of this pathway is GSK3β (glycogen syntase kinase3β). The main goal of this study was to evaluate GSK3β phosphorylation by treatment of cells with dihydroepiandrosterone (DHEA)، a kind of neurosteroid that decreases in the brain with aging. In this experimental study، neural progenitor cells were obtained from mouse embryos brain. Then، these cells were treated with 1µm concentration of DHEA for 48h. After 48h، the phosphorylation of GSK3β was analyzed by immunocytochemistry. DHEA increased phosphorylation of GSK3β in neural cells treated by DHEA، whole in control group، we could not detect the expression of GSK3β. DHEA can increase phosphorylation of GSK3β in neural cells and inactivation of GSK3β can help to cure AD.