The Effect of Daucus carota ssp. sativum seeds extract on serum levels of Renal Function Indicators and liver function enzymes in type I diabetes Model

With respect to antioxidant, antihyperglycemic and antihyperlipidemic properties of Daucus carota seeds extract in improvement diabetes mellitus, in this study, effects of this extract on renal function indicators and liver function enzymes were investigated in diabetic male rats. Diabete type 1 was induced in male wistar rats weighting 200-250g by injection of 70 mg/kg, i.p of streptozotocin. Before this and 5 days postinjection fasting blood samples were collected for measurement of serum levels of glucose and liver enzymes (AST, ALT) and kidney indicators(urea, creatinine, uric acid). Diabetes was confirmed in rats having FBS above 250 mg/dL. Diabetic animals were devided to 10 groups received 100, 200, 300 mg/kg extract, 0.5 mL distilled water and 600µg/kg glibenclamide daily for 3 and 14 days individually by gavage. After 3 and, 14 days rats sacrified and fasting blood samples were collected and above serum parameters were measured with commertial kits by spectrophotometery Administration of different doses of D. Carota seeds extract for 3 and 14 days did not increase serum levels of renal indicators or liver enzymes. Also administration of extract (300 mg/kg) for 3 days significantly decreased urea and for 14 days decreased creatinine serum level. Also administration of extract at doses 100, 300 mg/kg for 3 days decreased AST and at 200 mg/kg for 14 days decreased ALT serum levels. Using Daucus carota seeds extract for 3 or 14 days in remedy diabetes mellitus complications had no hepatotoxicity or renal damage.