The effect of basal media and hormonal treatment on Damask rose (Rosa damascene Mill.) micropropagation.

In vitro propagation of Damask rose via single nod explants was investigated on 3 different basal media، Murashige and Skoog (MS)، Lioyl and Cown (WP) and Quoirin and Lepoivre (QL). The effect of different basal media on shoot proliferation and media browning was studied. The highest percentage of proliferation was achieved on QL medium. Explants grown in the above media were transferred to the same media supplemented with BAP and Kn (1. 3 mg/l) for shoot proliferation. The highest shoot proliferation response and shoot growth was recorded when shoots were cultured in QL medium with 3 mg/l BAP. Comparison of different media (MS، WP، QL) based on number of shoot، leaves، leaflet number and shoot length indicate that QL medium was the best compared to other media for Damask rose in vitro micropropagation. Rooting of the regenerated shoots was induced on WP medium supplemented with 0. 2 mg/l NAA.