Preparation of Archaeosome Adjuvant from membrane Polar lipids of large scale cultured Methanobreveibacter smithii

Aim and Archaeal lipid derived liposomes referred to as Archaeosomes are among newly studied adjuvants with some specific immunopotent properties. In the present study large scale cultivation of Methanobrevibacter smithii, lipid extraction of obtained biomass and archaeosome production was assessed and evaluated. archaea Methanobrevibacter smithii, DSMZ 2375 was grown in the fermenter under controlled pH, temperature and anaerobic atmosphere condition to achieve archaeal cell mass. Total polar lipids were extracted via methanol/chloroform/water (2:1:0.8 v/v) solution from the frozen and thawed archaeal cells obtained from large scale cultivation. Archaeal liposomes prepared through hydration of extracted polar lipids by PBS buffer containing bovine serum albumin as the tentative antigen. Hydration was also carried out by BSA free PBS buffer to prepare antigen free archaeosomes to be compared with antigen containing type. Purity of extracted lipids evaluated via agarose gel electrophoresis and SDS-PAGE. Antigen concentration and entrapment analyzed by Lowry method and SDS-PAGE, respectively. Analysis of extracted lipids and archaeosomes revealed the high concentration and purity of obtained total polar lipids and successful entrapment of antigen in the archaeosomes. Adjuvant application of archaeosomes obtained from total polar lipids of Methanobrevibacter smithii could be a promising achievement in a variety of vaccine studies.