A comparison of recombinant proteins production in CHO and MCF 7 cell lines

The aim of the present study was to identify a suitable cell line for studies of recombinant protein production in eukaryotic system. After transfection, altered expression levels of RNA and its target protein were analyzed. To investigate the in vitro expression of E6 protein of human papillomavirus type 16 in cell culture, the plasmid pcDNA3-E6, and two different cell lines MCF7 and CHO were used. Following transfection, RNA expression was determined by RT- PCR and its protein target was evaluated by Western blot using SDS-PAGE gel and Anti-E6 monoclonal antibody. In this study due to the low concentration of proteins in MCF7cells, RT-PCR was positive and Western blotting was negative, while RT-PCR and Western blotting were positive for CHO cell lines. Regarding the results, the use of CHO expression system as a tool for efficient transfection of recombinant protein production is suggested.