Evaluation of CCND2, PLAB, PCSK2 and FHL1 Genes Expression Efficiency to Differentiate between Papillary Thyroid Carcinoma and Benign Tumors

Fine needle aspiration (FNA) is the best method for sample collection in diagnosis of thyroid tumors before surgery which is performed widely for patients, but unfortunately for about 20% of cases, the test result is reported as suspicious or intermediate. Also, due to false positive and negative responses, there is a pressing need for a biomarker to improve the accuracy of preoperative tests. The aim of this study was to evaluate the relative mRNA expression of genes CCND2, PLAB, PCSK2 and FHL1 in differentiating benign and malignant tumors of papillary thyroid carcinoma. Subjects and Fifty samples from patients with malignant thyroid tumors (25 cases) and benign thyroid tumors (25 cases) were enrolled in this study, RNA was extracted from paraffin samples with High Pure RNA Paraffin Kit and RNA extracted was used for cDNA synthesis. Real Time PCR reactions were performed in duplicate consists of 20 l of the reaction mixture containing the fluorescent cyber green. The relative mRNA expression of CCND2, PLAB and PCSK2 genes were not efficient for diagnosis. However, the relative mRNA expression of FHL1 gene could differentiate 21 malignant cases with 4 false positive results. The relative mRNA expression of FHL1 gene is more efficient to differentiate papillary thyroid carcinoma from benign tumors.