The Effect of Ethyl Methane Sulfonate on Some of Growth and Developmental Parameters in Plants Regenerated Petunia (Petunia hybrida Vilm.)

In order to evaluate the effect of Ethyl Methane Sulfonate (EMS) on some of growth and developmental parameter in regenerated plant of Petunia hybrida Vilm.، seeds of Petunia were cultured on MS medium. Then the large number of plant was reproduced from each single plant. Leaf and stem segments were treated with EMS 1% and 2% for 15، 30 and 60 minutes and were transferred to medium containing cytokinin BAP (benzyl amino purine). Finally photosynthetic pigments، diameter vascular system and root formation in regenerated plant were recorded. Protein from leaf segment was extracted and the total protein، and the protein patterns was evaluated using SDS-PAGE. The Results showed that treated plant with higher concentration of EMS for longer duration time، showed the significant differences in all parameters compared with control and other plants. These differences were similar with differences in protein assay and SDS-PAGE pattern of protein. However، the concentration and duration of EMS treatment resulted in change the plant is different for each plant and it is depending on the plant used for the study.