Correlation of C→T Polymorphism in -509 Promoter Region of TGF-β1 Gene with Long-Term Pulmonary Complications in Sulfur Mustard Chemical Injures

Sulfur mustard causes blister and was used for the first time as a chemical weapon in World War I and during the Iran-Iraq war as well. The aim of this study was to investigate the correlation of C→T polymorphism in -509 promoter region of TGF-β1 gene with long-term pulmonary complications in sulfur mustard chemical injures. This study was done on 252 chemically-injured veterans including 107 exposed subjects with pulmonary problems and 145 subjects without pulmonary problems. Peripheral blood samples were collected in the tubes containing EDTA. Then، genomic DNA was extracted of peripheral blood leukocytes. PCR-RFLP method was used to determine genotype of the subjects. Statistical analysis was done using SPSS 18 by Chi-square test. 33 subjects in chemically-injured patients with pulmonary problems group and 43 subjects in groups without pulmonary problems had CC homozygous genotype. 59 chemically-injured patients with pulmonary problems and 76 subjects of the group without pulmonary problems had CT heterozygous genotype (p=0. 967). Also، 15 subjects of pulmonary problems group and 26 subjects of the group without pulmonary problems had TT homozygous genotype (p=0. 473). No significant difference was observed between different genotype distribution of the polymorphism of C→T in -509 promoter region of TGF-β1 gene of with and without pulmonary problem groups. C→T polymorphism in -509 promoter region of TGF-β1 gene is not correlated with pulmonary problems in chemical injured veterans.